响应面法优化提取仿刺参中海参皂苷工艺

doi:10.16378/j.cnki.1003-1111.20116

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Abstract The process of saponin extracting from sea cucumber Apostichopus japonicus was optimized to provide reference for the quality evaluation. Cladoloside A was used for evaluation index, and three independent variable factors including microwave time, temperature and ethanol concentrations were examined. The extraction process of cladoloside A was optimized by single factor experiment coupled with Box-Benhken response surface method. Results showed that based on the regression result and considering the feasibility of experiment conduction, the following independent variable values were chosen as the optimized conditions for the extraction of saponin: extraction time of 10 min, extraction temperature of 45 ℃ and 25 mL 70% ethanol under 400 W. The response surface method was used to optimize the extraction of saponins, and it was feasible to provide technical support for the quality control of sea cucumber.

Key words： Apostichopus japonicus sea cucumber saponin microwave-assisted extraction response surface

Received: 08 June 2020

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	Articles by authors
	LIU Guiying
	WANG Xuda
	GE Kun
	HU Chaokui
	WU Jinhao
	LI Ai
	ZHENG Jie
	ZHOU Zunchun

Cite this article:

LIU Guiying,WANG Xuda,GE Kun, et al. Optimization of Microwave-Assisted Extraction Conditions for Saponins in Sea Cucumber Apostichopus japonicus by Response Surface Methodology[J]. Fisheries Science, 2021, 40(6): 810-817.

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https://www.shchkx.com/EN/10.16378/j.cnki.1003-1111.20116 OR https://www.shchkx.com/EN/Y2021/V40/I6/810

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