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| Differences in Intestinal Morphology and Enzymatic Characteristics between Koi and Goldfish in Hybrid and Self-Crossed Offsprings |
| LIU Junheng, FENG Xiaosa, ZHANG Yuan, GAO Jianzhong |
1. National Experimental Teaching Demonstration Center of Fisheries Science, Shanghai Ocean University, Shanghai 201306, China;
2. Shanghai Aquaculture Engineering Technology Research Center, Shanghai Ocean University, Shanghai 201306, China;
3. Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, China |
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Abstract By comparing the intestinal characteristics of the orthogonal, reciprocal and self cross offsprings of Showa koi Cyprinus carpio and long tail grass goldfish Carassius auratus, we hope to produce and study the distant hybridization of koi and goldfish to provide theoretical basis. In this study, the intestinal parameters of the orthogonal, reciprocal, and self-crossed progeny of the Showa koi and the long tail goldfish were measured, and the morphological differences were analyzed; then the pH and digestion of the intestinal environment of the F1 progeny were measured and compared enzyme activity. The results showed that the reciprocal F1 generations were in intermediate between the parental inbred F1 generations in relative intestinal length, relative intestinal density, relative intestinal mass, and Zihler′s index; and the pH of the foregut was also in intermediate between Showa koi and long tail grass goldfish self-breeding between F1 generations; however, the intestinal amylase and lipase activities of reciprocal F1 generations were significantly higher than those of self-breeding F1 generations, while protease activity was in intermediate between self-breeding F1 generations. The intestinal parameters of the progeny and the reciprocal progeny of the Showa koi and long tail goldfish were higher than or between those of the two self-crossed progeny, and the intestinal traits of the two were integrated.
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Received: 28 February 2020
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