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| Transcriptome Analysis of Transparent Skin in Grass GoldfishCarassius auratus |
| LIU Xiaolian, LI Chunyan, BAI Xiaohui, HAO Shuang, LIU Guoshan, LIU Keming, MA Lin, JIANG Jufeng |
| Tianjin Ornamental Fish Technology and Engineering Center, Tianjin Fisheries Research Institute, Tianjin 300221, China |
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Abstract Two libraries of transparent group and normal group were constructed and sequenced in grass goldfish Carassius auratus with body length of (45.00±5.42) mm by Illumina high-throughput sequencing technology to explore the functional genes related to transparent trait of grass goldfish. A total of 309 364 078 clean reads were generated from six skin tissues, and assembled into 72 083 unigenes with an average length of 1321 bp. DESeq analysis identified 181 differentially expressed genes (DEGs) between the two groups, among which 66 genes were up-regulated and 115 genes were down-regulated. Gene ontology functional enrichment analysis showed that DEGs were mostly involved in microtubule, microtubule cytoskeleton organization, intermediate filament, myosin complex, protein binding, guanyl ribonucleotide binding, pigmentation, and pigment granule transport. KEGG pathway analysis revealed that DEGs were mainly distributed in MAPK signaling pathway, regulation of actin cytoskeleton, purine metabolism, and protein processing in endoplasmic reticulum. In addition, 38 775 microsatellite loci were screened out from 72 083 unigenes with the highest frequency of mono-nucleotide repeats (53.95%) and followed by di-nucleotide repeats (24.45%). The finding provides basic information for exploring new genes related to transparent trait and their functional analysis of grass goldfish C. auratus.
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Received: 26 August 2020
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