不同来源杀鲑气单胞菌毒力因子的比较基因组学研究

doi:10.16378/j.cnki.1003-1111.20199

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Abstract A pathogenic Aeromonas salmonicida strain YK48 was isolated from the water of the Pacific white shrimp culture ponds. We first tested the physiological characteristics and antimicrobial susceptibility of strain YK48. Afterwards, to obtain its virulence profile, strain YK48 was sent for whole-genome sequence. Together with one strain isolated from goldfish Carassius auratus in Anshan and another two isolated from Yantai, a comparative genomic analysis was conducted to determine whether the strains had distinct virulence gene profile and antibiotic resistance genes. Phylogenetic analysis revealed that AS.17 and YK-48 were clustered together with Aeromonas salmonicida ssp. salmonicida strain A449; antimicrobial susceptibility test showed that strain YK-48 was resistant to rifampicin, bacitracin, vancomycin and penicillin; comparative genome analysis showed that the virulence genes of strains AS.17 and YK-48 were basically identical. Compared with S68 and S121, YK-48 and AS.17 lacked the genes related to anti-phagocytosis and immune regulation. Adherence assays also verified that strain YK-48 and AS.17 had relative weak adhesion ability. At present, there was comparative genomic analysis on the virulence factors of different A. salmonicida from distinct sources. The A. salmonicida strains from three sources were compared through comparative genomics, which provided valuable information regarding the pathogenicity of A. salmonicida, and provided useful insights into the prevention and control of the disease.

Key words： Aeromonas salmonicida antibiotic resistance gene virulence gene insertion sequence phylogenetic relationship

Received: 02 September 2020

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	Articles by authors
	HAO Jingwei
	WANG Yi
	HE Fenglan
	FU Songzhe
	SHEN Xudong
	LIU Ying

Cite this article:

HAO Jingwei,WANG Yi,HE Fenglan, et al. Genomic Characteristics of Virulence Factors of Aeromonas salmonicida Strains from Distinct Sources[J]. Fisheries Science, 2022, 41(2): 192-201.

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https://www.shchkx.com/EN/10.16378/j.cnki.1003-1111.20199 OR https://www.shchkx.com/EN/Y2022/V41/I2/192

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