盐度对青海湖裸鲤生长及渗透调节基因的影响

doi:10.16378/j.cnki.1003-1111.21077

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Abstract Qinghai Lake is the largest inland brackish lake in China. The salinity adaptation mechanism of Gymnocypris przewalskii, a unique and rare fish species living in it, needs to be clarified urgently. Therefore, the growth performance and osmotic regulation genes of G. przewalskii were investigated after salinity stress by physiological index and qPCR and Na⁺/K⁺/2Cl^- cotransporter 1 (NKCC1), Aquaporin 1 (AQP1), Na⁺/H⁺ exchange 1 (NHE1) and Na⁺/HCO^-₃ exchange 1 (NBC1) genes were detected. The results showed that there were significant differences in the growth indicators of G. przewalskii under different salinities (P<0.05), indicating that the increase in salinity affected the development of G. przewalskii, and that the growth status were negatively correlated with salinity. NKCC1, NBC1, AQP1 and NHE1 genes were expressed in the gills, kidneys and livers under different salinities, showing certain tissues specificity. It was found that that NKCC1, AQP1, NHE1 and NBC1 genes were involved in the regulation of osmotic pressure of G. przewalskii, with varying the expression level with different salinities, tissue and stress time, reflecting that the gill and kidney were the main organ regulating osmotic pressure. The findings provided a theoretical basis for exploring the survival status and salinity adaptation mechanism of G. przewalskii after migration in Qinghai Lake, and a theoretical guidance for the protection and artificial proliferation and release of native fish germplasm resources in the upper reaches of the Yellow River.

Key words： Gymnocypris przewalskii salinity growth performance molecular response

Received: 07 May 2021

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	Articles by authors
	HUANG Shen
	LI Changzhong
	LI Zixuan
	LIU Yanhui
	LIU Fang
	QI Hongfang
	WANG Yang
	JIN Wenjie

Cite this article:

HUANG Shen,LI Changzhong,LI Zixuan, et al. Effects of Different Salinities on Growth and Osmotic Regulation Gene Expression of Gymnocypris przewalskii[J]. Fisheries Science, 2022, 41(4): 527-536.

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https://www.shchkx.com/EN/10.16378/j.cnki.1003-1111.21077 OR https://www.shchkx.com/EN/Y2022/V41/I4/527

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