斑点叉尾鮰肠道及其养殖环境菌群结构分析

doi:10.16378/j.cnki.1003-1111.20175

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Abstract In order to study the structure of intestinal microflora of healthy channel catfish and its relationship with pond culture water, the composition and diversity of microbial communities were comparatively investigated in the intestinal tract and culture water by 16S rDNA high-throughput sequencing and bioinformatics analysis method. The results showed that the abundance and diversity of intestinal flora of channel catfish were lower than those of culture water through diversity index analysis. 69.57% of OTUs in channel catfish was consistent with that in water. However, the dominant microorganisms in the water were Proteobacteria, Actinobacteria and Cyanobacteria, while the dominant microfloras in the intestinal tract were Bacteroidetes, Fusobacteria and Firmicutes. There was no significant correlation between the composition and abundance of the dominant microflora in the intestinal tract and the culture water. The composition of the intestinal microflora had certain independence, and its dominant bacterial species were almost independent of the aquaculture environment. PICRUSt prediction of the bacterial phenotype revealed that anaerobes and Gram-positive bacteria were the main bacteria in the intestinal tract of channel catfish. The biofilm formation and pathogenicity potential phenotype showed that there were a large number of opportunistic pathogens in the intestinal tract and water. In this study, the average relative abundances of Cetobacterium and Bacteroides in intestinal flora of channel catfish were 36.60% and 15.82%, indicating that the dominant probiotics in intestinal microorganism of channel catfish were mainly Cetobacterium and Bacteroides. The finding provides theoretical basis for healthy breeding of channel catfish and screening of probiotics.

Key words： Ictalurus punctatus intestinal flora high-throughput sequencing microbial community structure

Received: 14 August 2020

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	Articles by authors
	XIONG Xiangying
	ZHAO Yanfei
	WANG Zhicheng

Cite this article:

XIONG Xiangying,ZHAO Yanfei,WANG Zhicheng. Analysis of Microbial Community Structure of Channel Catfish Ictalurus punctatus Intestine and Culture Water[J]. Fisheries Science, 2022, 41(4): 589-596.

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https://www.shchkx.com/EN/10.16378/j.cnki.1003-1111.20175 OR https://www.shchkx.com/EN/Y2022/V41/I4/589

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