凡纳滨对虾LvML1基因克隆与表达分析

doi:10.16378/j.cnki.1003-1111.21182

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Abstract ML (MD-2-related lipid-recognition) genes are a class of proteins with important functions in innate immunity and lipid metabolism in invertebrates. In this study, we cloned a novel ML gene from Pacific white shrimp Litopenaeus vannamei named LvML1. The full-length cDNA sequence of LvML1 consists of a 254 bp 5'-untranslated regions (5'-UTR), a 913 bp 3'-UTR and a 462 bp open reading frame (ORF). The deduced amino acid sequence is composed of 153 amino acids. LvML1 has seven cysteine residues, which are predicted to form three pairs of disulfide bonds, with a typical ML family structural domain. Multiple sequence alignment revealed that both the ML structural domain and cysteine residue positions were relatively conserved. Phylogenetic tree showed that LvML1 clustered with ML proteins of crustaceans including tiger shrimp Penaeus monodon, kuruma shrimp Marsupenaeus japonicus and spiny lobster Homarus americanus. Real-time quantitative PCR results showed that LvML1 was expressed in hepatopancreas, eyestalk, intestine, stomach, heart, gill, muscle and blood, with the highest expression level in the heart. Expression in larval developmental stages showed that LvML1 was already expressed from the nauplius of Pacific white shrimp and was upregulated at each metamorphosis transition to zoea, mysis and postlarva, indicating that it is involved in the larval developmental processes of shrimp. Expression of LvML1 in hepatopancreas and gills exposed to artificial injection of Decapod iridescent virus 1(DIV1) for 0 h and 24 h was significantly down-regulated with P<0.01, indicating that it is involved in the regulation of innate immunity against the virus in shrimps. The finding provides basic data for understanding of the structure and function of the ML protein family in Pacific white shrimp and its important role in the innate immunity and larval developmental processes of the shrimp.

Key words： Litopenaeus vannamei MD-2-related lipid-recognition (ML) gene tissue expression developmental expression Decapod iridescent virus 1(DIV1)

Received: 10 September 2021

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	LIU Hongtao
	WANG Kun
	YANG Mingqiu

Cite this article:

LIU Hongtao,WANG Kun,YANG Mingqiu. Molecular Cloning and Expression Analysis of LvML1 Gene in Pacific White Shrimp Litopenaeus vannamei[J]. Fisheries Science, 2023, 42(2): 175-184.

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https://www.shchkx.com/EN/10.16378/j.cnki.1003-1111.21182 OR https://www.shchkx.com/EN/Y2023/V42/I2/175

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