不同环境因素对虾肝肠胞虫感染脊尾白虾的影响研究

doi:10.16378/j.cnki.1003-1111.20280

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Abstract The spread of Enterocytozoon hepatopenaei (EHP) is related to the host physique and water environment. Environmental factors such as temperature, salinity, and ammonia nitrogen concentration are extremely important influencing factors in the breeding process. It is shown that too high or too low temperature and salinity have a greater impact on the specific growth rate and non-specific immunity of ridgetail prawn Exopalaemon carinicauda. In this study, the EHP capacity in ridgetail prawn was used as an indicator to determine the transmission rate of EHP. The interaction of temperature, salinity and ammonia nitrogen on the transmission of EHP were discussed. In this experiment, three factors and three levels of orthogonal culture experiment were designed, including temperature (22 ℃, 24 ℃, 26 ℃), salinity (20, 24, 28) and ammonia nitrogen concentration (2 mg/L, 4 mg/L, 6 mg/L). Through a ten-day artificial infection experiment, real-time fluorescence quantitative PCR was used to detect EHP load under different environmental factors. The results showed that under the experimental conditions, the EHP capacity increased significantly (P<0.05). The influence of environmental factors on EHP transmission in the shrimp was temperature, ammonia nitrogen and salinity. The optimum propagation conditions were temperature 24 ℃, ammonia nitrogen 2 mg/L and salinity 28, and the unsuitable conditions were temperature 26 ℃, ammonia nitrogen 4 mg/L and salinity 20. The findings ascertained the environmental conditions for the transmission of intestinal worms in shrimp, and provided data reference for the prevention and control of intestinal cysts in shrimp in marine aquaculture.

Key words： environmental factor Enterocytozoon hepatopenaei Exopalaemon carinicauda capacity transmission condition

Received: 17 December 2020

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	Articles by authors
	DUAN Jiancheng
	HU Jihui
	MU Jinxin
	DENG Gaowei
	GAO Wei
	MU Hua
	ZHANG Qingqi
	GAO Huan

Cite this article:

DUAN Jiancheng,HU Jihui,MU Jinxin, et al. Effects of Different Environmental Factors on Infection of Enterocytozoon hepatopenaei in Ridgetail White Prawn Exopalaemon carinicauda[J]. Fisheries Science, 2023, 42(2): 296-302.

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https://www.shchkx.com/EN/10.16378/j.cnki.1003-1111.20280 OR https://www.shchkx.com/EN/Y2023/V42/I2/296

[1]梁俊平,李健,刘萍,等.脊尾白虾生物学特性与人工繁育的研究进展[J].中国农学通报,2012,28(17):109-116.
[2]RAJENDRAN K V, SHIVAM S, PRAVEENA P E, et al. Emergence of Enterocytozoon hepatopenaei (EHP) in farmed Penaeus (Litopenaeus) vannamei in India[J].Aquaculture,2016,454:272-280.
[3]乔毅,沈辉,万夕和,等.南美白对虾肝肠胞虫的分离及形态学观察[J].中国水产科学,2018,25(5):1051-1058.
[4]SANTHOSHKUMAR S, SIVAKUMAR S, VIMAL S, et al. Biochemical changes and tissue distribution of Enterocytozoon hepatopenaei (EHP) in naturally and experimentally EHP-infected whiteleg shrimp, Litopenaeus vannamei (Boone, 1931), in India[J].Journal of Fish Diseases,2017,40(4):529-539.
[5]ARANGUREN L F, HAN J E, TANG K F J. Enterocytozoon hepatopenaei (EHP) is a risk factor for acute hepatopancreatic necrosis disease (AHPND) and septic hepatopancreatic necrosis (SHPN) in the Pacific white shrimp Penaeus vannamei[J].Aquaculture,2017,471:37-42.
[6]王元,房文红,周俊芳,等.脊尾白虾肌肉微孢子虫病的病原和病理分析[J].上海海洋大学学报,2013,22(5):726-733.
[7]李志辉,王玉,李光光,等.温度和盐度对脊尾白虾生长和非特异性免疫的影响[J].海洋湖沼通报,2019(6):78-82.
[8]王兴强,曹梅.低盐和低温对脊尾白虾生长和能量收支的影响[J].水生态学杂志,2010,31(2):66-71.
[9]赖晓芳,沈善瑞,吕海波.水温和盐度对脊尾白虾氮磷代谢的影响[J].江苏农业科学,2013,41(11):258-259.
[10]蒋玫,沈新强,李磊,等.氨氮对脊尾白虾幼体存活生长及体内RNA/DNA比值的影响[J].动物学杂志,2011,46(4):102-108.
[11]LU X, KONG J, LUAN S, et al. Transcriptome analysis of the hepatopancreas in the Pacific white shrimp (Litopenaeus vannamei) under acute ammonia stress[J].PLoS One,2016,11(10):e0164396.
[12]任海,李健,李吉涛,等.急性氨氮胁迫对脊尾白虾(Exopalaemon carinicauda)抗氧化系统酶活力及GPx基因表达的影响[J].农业环境科学学报,2014,33(4):647-655.
[13]梁俊平,李健,李吉涛,等.氨氮对脊尾白虾幼虾和成虾的毒性试验[J].水产科学,2012,31(9):526-529.
[14]VU-KHAC H, THI THANH T N, THI THU G N, et al. Vertical transmission and early diagnosis of the microsporidian Enterocytozoon hepatopenaei in whiteleg shrimp Penaeus vannamei[J].Journal of Pure and Applied Microbiology,2018,12(3):1125-1131.
[15]SALACHAN P V, JAROENLAK P, THITAMADEE S, et al. Laboratory cohabitation challenge model for shrimp hepatopancreatic microsporidiosis (HPM) caused by Enterocytozoon hepatopenaei (EHP)[J].BMC Veterinary Research,2017,13(1):9.
[16]SINGH M, SINGH P. Enterocytozoon hepatopenaei: a microsporidian in the midst of serious threat to shrimp aquaculture[J]. Entomology and Zoology Studies, 2018, 6(6): 936-939.
[17]刘珍,张庆利,万晓媛,等.虾肝肠胞虫(Enterocytozoon hepatopenaei)实时荧光定量PCR检测方法的建立及对虾样品的检测[J].渔业科学进展,2016,37(2):119-126.
[18]叶键,许婷,施礼科,等.3种虾肝肠胞虫PCR检测方法的应用比较分析[J].水产科学,2019,38(3):411-415.
[19]陈禄芝,余霞艳,胡一丞,等.粤西地区凡纳滨对虾虾肝肠胞虫、传染性皮下和造血组织坏死病毒感染情况的初步调查[J].渔业研究,2016,38(4):273-280.
[20]程东远,邱亮,宋增磊,等.凡纳滨对虾感染虾肝肠胞虫的群体及组织间差异性分析[J].渔业科学进展,2018,39(4):83-92.
[21]冼健安,钱坤,郭慧,等.氨氮对虾类毒性影响的研究进展[J].饲料工业,2014,35(22):52-58.
[22]姜巨峰,吴会民,郭华阳,等.盐度对脊尾白虾生长和肌肉营养成分的影响[J].渔业现代化,2014,41(3):15-20.
[23]WIREDU BOAKYE D, JAROENLAK P, PRACHUMWAT A, et al. Decay of the glycolytic pathway and adaptation to intranuclear parasitism within Enterocytozoonidae microsporidia[J].Environmental Microbiology,2017,19(5):2077-2089.
[24]丁慧昕,施慧,谢建军,等.虾肝肠胞虫(EHP)在凡纳滨对虾养殖水环境中的分布情况及传播途径初步研究[J].浙江海洋大学学报(自然科学版),2018,37(1):14-19.
[25]徐胜威,杨程,斯烈钢,等.氨氮胁迫对凡纳滨对虾的生长状况及虾肝肠胞虫(EHP)携带量的影响[J].安徽农业科学,2019,47(7):109-112.