|
|
|
| Substance Exchange between Water-Vascular System and Coelom in Sea Cucumber Apostichopus japonicus |
| FAN Xuyuan1,2, WANG Zhenhui2, REN Yuan2,3, GUO Liyuan1,2, SUN Qirui1,2, WANG Yinan2, LI Qiang2 |
1. Dalian Ocean University, Dalian 116023, China;
2. College of Marine and Biology Engineering, Yancheng Institute of Technology, Yancheng 224051, China;
3. School of Bioengineering, Dalian University of Technology, Dalian 116024, China |
|
|
|
|
Abstract Sea cucumber Apostichopus japonicus with a body mass of (65±10) g was injected in coelom with beet root pigments—beetroot red, purified and adjusted GST-tagged protein, fluorescent microsphere dilution, suspension of Vibrio splendidus and suspension of Escherichia coli. The distribution of the injected matters in the coelom and the water-vascular system (represented by polian vesicle) was detected to explore the correlation, connectivity and material exchange between the coelom and the water-vascular system of the sea cucumber. The results showed that the coelomic fluid from coelom and polian vesicle (hereinafter referred to as “vesicle fluid”) were stained into red at 6 hours after injection by beet red dye. A large number of GST-tag proteins were detected in the coelomic fluid, and there were also some proteins in the vesicle fluid 6 h and 12 h after protein injection. Many fluorescent microspheres were observed in the coelomic fluid, but not in the vesicle fluid 6 h and 12 h after injection. In addition, a large number of E. coli and V. splendidus bacteria were found in the coelomic fluid 6, 12, 24 and 72 h after injection, without bacteria isolated in the vesicle fluid. In conclusion, the water-vascular system and the coelom are not found to be infinitely unblocked in sea cucumber. Soluble substances such as small molecules and biological macromolecules were exchanged between the coelom and the water-vascular system, but the exogenous particles and bacteria did not enter the water-vascular system via the coelom. The findings laid a theoretical foundation for the subsequent study of the physiological structure and biological role of the water-vascular system in sea cucumber.
|
|
Received: 25 September 2021
|
|
|
|
|
|
[1]DORNBOS S Q. Evolutionary palaeoecology of early epifaunal echinoderms:response to increasing bioturbation levels during the Cambrian radiation[J].Palaeogeography, Palaeoclimatology, Palaeoecology,2006,237(2/3/4):225-239.
[2]GROSS P S, AL-SHARIF W Z, CLOW L A, et al. Echinoderm immunity and the evolution of the complement system[J].Developmental & Comparative Immunology,1999,23(4/5):429-442.
[3]陈仲,蒋经伟,高杉,等.不同病原菌刺激后仿刺参幼参体腔细胞中免疫相关酶的应答变化[J].水产科学,2018,37(3):295-300.
[4]肖瑶,蒋经伟,李石磊,等.仿刺参成参体腔细胞酚氧化酶的生化与酶学特性研究[J].水产科学,2021,40(4):516-522.
[5]DORIT R L, WALKER W F, BARNES R D. Zoology[M].Philadelphia:Saunders College Pub,1991:788-789.
[6]BARNES R D. Invertebrate Zoology[M].Philadelphia:Saunders College Pub,1980:981-996.
[7]NICHOLS D. The water-vascular system in living and fossil echinoderms[J].Palaeontology,1972,15(4):519-538.
[8]LI Q, QI R R, WANG Y N, et al. Comparison of cells free in coelomic and water-vascular system of sea cucumber, Apostichopus japonicus[J].Fish & Shellfish Immunology,2013,35(5):1654-1657.
[9]REN Y, ZHANG J L, WANG Y N, et al. Non-specific immune factors differences in coelomic fluid from polian vesicle and coelom of Apostichopus japonicus, and their early response after evisceration[J].Fish & Shellfish Immunology,2020,98:160-166.
[10]杨丹,叶佩莹,万津,等.谷胱甘肽S-转移酶的诱导表达及提纯[J].氨基酸和生物资源,2004,26(3):33-37.
[11]WANG S X, XU L, WANG X L, et al. Expression of immune-related genes in sea cucumber(Apostichopus japonicus) during bacterial challenge[J].Agricultural Science & Technology,2016,17(4):1024-1027.
[12]廖玉麟.中国动物志:棘皮动物门 海参纲[M].北京:科学出版社,1997:1-47.
[13]YANG H S, MERCIER J F H A A. The sea cucumber Apostichopus japonicus:history, biology and aquaculture[M].Amsterdam: Academic Press,2015:53-76.
[14]LI Q, REN Y, LUAN L L, et al. Localization and characterization of hematopoietic tissues in adult sea cucumber, Apostichopus japonicus[J].Fish & Shellfish Immunology,2019,84:1-7.
[15]GUO L Y, WANG Z H, SHI W B, et al. Transcriptome analysis reveals roles of polian vesicle in sea cucumber Apostichopus japonicus response to Vibrio splendidus infection[J].Comparative Biochemistry and Physiology Part D:Genomics and Proteomics,2021,40:100877.
[16]SMITH A C. A proposed phylogenetic relationship between sea cucumber Polian vesicles and the vertebrate lymphoreticular system[J].Journal of Invertebrate Pathology,1978,31(3):353-357.
[17]SMITH A C. Immunopathology in an invertebrate, the sea cucumber, Holothuria cinerascens[J].Developmental & Comparative Immunology,1980,4:417-431.
[18]王萍,闫明哲.红甜菜色素稳定性影响因素研究进展[J].食品与生物技术学报,2021,40(7):19-29.
[19]KIRYU I, OTOTAKE M, NAKANISHI T, et al. Transfer of fluorescent microspheres from the integumental tissues to the kidney and spleen in rainbow trout[J].Fish Pathology,2000,35(3):165-166.
[20]孟繁伊.仿刺参(Apostichopus japonicus, Selenka)体腔液中调理素样分子的研究[D].青岛:中国海洋大学,2009:48-69.
[21]WANG Z H, LV Z M, LI C H, et al. An invertebrate β-integrin mediates coelomocyte phagocytosis via activation of septin2 and 7 but not septin10[J].International Journal of Biological Macromolecules,2018,113:1167-1181.
[22]JIANG L T, SHAO Y N, XING R L, et al. Identification and characterization of a novel PRR of fibrinogen-related protein in Apostichopus japonicus[J].Fish & Shellfish Immunology,2018,82:68-76. |
|
|
|
