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| Modified Atmosphere Preservation-Alive Technology of Hybrid Grouper Epinephelus fuscoguttatus ♀×E. lanceolatus ♂ |
| HUANG Xiangmei, HUANG He, ZHOU Qianfan, LU Zefeng |
| Key Laboratory of Advanced Processing of Aquatic Products of Guangdong Higher Education Institution, Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, China |
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Abstract In order to explore the effects of preservation conditions on the waterless preservation time of hybrid grouper Epinephelus fuscoguttatus ♀×E. lanceolatus ♂, a single factor test was carried out to screen the optimal range of conditions by using the proportion of O2, CO2 and N2 gases, survival temperature and moisturizing materials, and orthogonal experiment was conducted to optimize the preservation conditions of atmosphere preservation-alive. The muscle components and blood biochemical indices in different survival stages were determined. The results showed that the maximal waterless survival period was observed under the gas ratio of V(O2)∶V(CO2)∶V(N2)=85∶0∶15, the preservation temperature of 15 ℃, and the moisturizing material of sponge, with the 12.5 h survival rate of 100%. The weight loss rate of hybrid grouper was increased gradually with the prolongation of survival time, and the cumulative weight loss rate reached (6.49±0.15)% after rehydration for 12 h. There was no significant change in moisture content in dorsal muscle(P＞0.05), but the ash content was slightly increased. The contents of crude protein and crude fat were gradually decreased, and the decrease of fat content during the whole process was much greater than that of protein, which might be because the main energy supply substance in the body of the hybrid grouper was fat rather than protein during the period of atmosphere preservation-alive and maintenance. The blood glucose and cortisol contents showed curve changes with the prolonging of survival time. The contents of BUN, AST, ALT and ACP were significantly increased, while the contents of TG and AKP were decreased. In conclusion, the kidney, liver and immune system of the hybrid grouper were all damaged to a certain extent during atmosphere preservation-alive, but they all recovered after resuscitation, and the survival time was longer than that in pure oxygen environment. Therefore, the optimal atmosphere preservation-alive conditions selected were conducive to the waterless preservation transportation of grouper. The finding will provide theoretical support with the practical application of atmosphere preservation-alive and preservation transportation of grouper.
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Received: 31 August 2021
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