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| Molecular Cloning of agrp Genes and Their Expression Pattern in Scatophagus argus under Fasting and Refeeding |
| CAI Bosheng1,2, CHEN Huahui1, LUO Cuiting1, ZHAI Yi1,2, LI Guangli1,2, DENG Siping1,2 |
1. Fisheries College, Guangdong Ocean University, Zhanjiang 524088, China;
2. Guangdong Research Center on Reproductive Control and Breeding Technology of Indigenous Valuable Fish Species, Zhanjiang 524088, China |
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Abstract To investigate the roles of agouti-related protein/neuropeptide Y (AgRP) neurons in response to starvation and refeeding in spotted scat Scatophagus argus, Agrp1 and agrp2 genes were cloned from the hypothalamus of spotted scat by RT-PCR. The expression level of the two genes in different tissues was detected by qPCR. The expression of agrp1 and agrp2 genes in the hypothalamus, liver and intestine of the normal feeding group (2 d, 7 d), starvation group (2 d, 7 d), and refeeding group (7 d starvation followed by refeeding for 3 h) of spotted scat (body weight: 52—60 g) was investigated. Sequence analysis showed that the open reading frames of agrp1 and agrp2 genes were 429 bp and 351 bp, respectively. Agrp1 gene was mainly expressed in hypothalamus and muscle,whereas agrp2 gene was mainly expressed in hypothalamus and pituitary. No significant change was observed in the expression level of agrp1 gene in hypothalamus after 2 d of food deprivation. However, it decreased after 7 d of food deprivation and then increased to the normal level after refeeding. In liver, the expression level of agrp1 gene was decreased after 2 d of food deprivation, but increased after 7 d of food deprivation andthendecreased to the normal level after refeeding. In intestine, the expression level of agrp1 gene did not change after 2 d of food deprivation. However, it increased significantly after 7 d of food deprivation and then decreased to the same levels of the control group after refeeding.In terms of the expression level of agrp2 gene in the hypothalamus, liver and intestines of the starved group, there was no significant difference comparing with the control group during the fast feeding and refeeding period. In summary, the agrp1 gene might be involved in the regulation of food intake in hypothalamus, liver and intestine in spotted scat.
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Received: 19 August 2021
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