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| Construction of Zebrafish Danio rerio Enteritis Model Induced by Dextran Sodium Sulfate |
| MA Jun1,2,3, CHEN Yan1,2,3, LIU Lu1, HOU Guoyu1, HU Chengzheng1, WAN Xinming1, CHEN Pan1,2,3, LI Wanting1, HUANG Hai1,2,3 |
1. Hainan Tropical Ocean University, Sanya 572022, China;
2. Key Laboratory of Tropical Marine Fishery Resources Protection and Utilization of Hainan Province, Sanya 572022, China;
3. Key Laboratory of Utilization and Conservation for Tropical Marine Bioresources, Ministry of Education, Sanya 572022, China |
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Abstract The intestinal inflammation model in adult zebrafish can provide a better powerful experimental platform for the pathogenesis of intestinal inflammatory in economically important fish and the screening of therapeutic drugs. In this study, dextran sodium sulfate (DSS, 5%) was injected into adult zebrafish Danio rerio by oral and anal injection. There was slightly higher survival rate in oral injection group than that in anal injection group, the vitality of fish becoming to be recovery on the third day after induction. At this time, histological analysis showed that the zebrafish in the oral injection group induced mild inflammation, while the zebrafish in the ones in the anal injection group were induced mild and moderated inflammation. The oral injection was simpler and more efficient and selected to dynamically monitor the effects of DSS on intestinal tissue and myeloperoxidase (MPO) activity in adult zebrafish, though oral and anal injection produced similar inflammatory characters. The foregut and mid-gut were more sensitive to DSS by oral injection, on the 1st and 3rd days after induction, with obvious local intestinal wall thickening and eosinophil infiltration. The caudal intestine was relatively slow response to the sodium dextran sulfate (DSS), the change of intestinal wall was not obvious, and the eosinophil infiltration and aggregation appeared on the 6th and 9th days after induction. In addition, epithelial fold disruption or shedding was common, without obvious correlation with DSS induction. The number of goblet cells in the intestinal mucosal layer was inflected by a certain stress response to DSS induction. On the 1st and 3rd days after induction, the number of goblet cells in the foregut, midgut and hindgut was increased significantly and gathered on the surface of the mucosal layer, and the change trend of the number of goblet cells was consistent with the myeloperoxidase (MPO) activity of the intestinal tissue.
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Received: 18 September 2021
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