棘胸蛙胚胎发育观察与相关抗氧化酶活性变化研究

doi:10.16378/j.cnki.1003-1111.21209

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Abstract In order to investigate the changes in morphological character and autioxidant enzyme activities during the embryo development of giant spiny frog Quasipaa spinosa, the cultured breeding parents were prepared to mature and spawn in imitation ecology condition. The whole process of embryonic development was continuously observed, photographed, and measured. The activities of autioxidant enzymes were identified follow the instructions. The fertilized egg was belong to the sinuous viscous egg with (4.31±0.69) mm in length. The results showed that under an incubation temperature of (24.5±0.5) ℃, it took 222.94 h for the whole embryonic development to hatching stage and 288.44 h to yolk exhausted stage with the total accumulative temperature of 470.95 h·℃ and 7078.32 h·℃, respectively. According to the external morphology and typical characteristics of embryonic development, it can be divided into seven stages. The activities of superoxide dismutase, catalase and glutamic-pyruvic transaminase showed the same trend as well as the malondialdehyde content significantly decreased in the mid-gastrula stage. The activity of glutamic oxalacetic transaminase was reached a peak in the same time, then showed continuous declination. In addition, the activities of catalase, superoxide dismutase glutamic-pyruvic transaminase and glutamic-pyruvic transaminase were minimal in the yolk absorbs stage, while the lowest levels of malondialdehyde was observed in the mid-gastrula stage. The finding will provide basic data and important references for seed production technology efficiently in the future.

Key words： Quasipaa spinosa embryonic development morphological characteristic antioxidase

Received: 22 October 2021

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	Articles by authors
	XIE Yongguang
	WANG Xiaodong
	WU Yafeng
	HUANG Fujiang
	WEI Xiuying
	WEI Zhaoyu
	CHEN Dunxue

Cite this article:

XIE Yongguang,WANG Xiaodong,WU Yafeng, et al. Embryonic Observation and Activities of Antioxidase in Giant Spiny Frog Quasipaa spinosaduring Embryonic Development[J]. Fisheries Science, 2023, 42(6): 1054-1062.

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https://www.shchkx.com/EN/10.16378/j.cnki.1003-1111.21209 OR https://www.shchkx.com/EN/Y2023/V42/I6/1054

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