不同质量浓度HgCl<sub>2</sub>对凡纳滨对虾的急性毒性评估

doi:10.16378/j.cnki.1003-1111.22007

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Abstract A study was carried out to test the effect of acute mercuric chloride (HgCl₂) exposure on Pacific white shrimp Litopenaeus vannamei. In order to investigate the acute toxic effect of HgCl₂ on Pacific white shrimp, the juvenile Pacific white shrimp with body weight of (5.01±0.06) g was placed in a 40 L glass tank (54 cm× 40 cm× 30 cm) at a water temperature of 27—29 °C at a rate of 40 individuals per tank and exposed to HgCl₂ at concentration of < 0.001 mg/L as the control group, 0.033 mg/L and 0.33 mg/L group. The plasma antioxidant enzyme activity, total antioxidant capacity and immune response indices and Hg content in the muscle were determined at 24 h, 48 h, 72 h and 96 h. The results showed that there was the maximal Hg content in muscle in the shrimp in 0.33 mg/L HgCl₂ group after 24 h (P>0.05). There were higher total antioxidant capacity and superoxide dismutase activity in plasma of shrimp in 0.033 and 0.33 mg/L HgCl₂ groups than those in shrimp in control group at 24 h (P>0.05). The lower superoxide dismutase and catalase activities were observed in plasma of shrimp in 0.33 mg/L HgCl₂ group than those in shrimp in 0.033 mg/L HgCl₂ group after 72 h, with the maximal malondialdehyde content (P>0.05). The maximal activities of acid phosphatase, alkaline phosphatase, lysozyme and phenol oxidase were shown in plasma of shrimp in 0.33 mg/L HgCl₂ group at 24 h (P<0.05). In 48 h, the acid phosphatase and lysozyme activities in plasma of shrimp in 0.33 mg/L HgCl₂ group were found to be the minimum (P<0.05). The contents of interleukin 1 and 8 in plasma of shrimp in 0.33 mg/L HgCl₂ group were the maximal after 24 h (P<0.05). The findings showed that the Pacific white shrimp exposed to HgCl₂ led to accumulation of Hg in muscle and malondialdehyde in blood, reducing antioxidant enzyme activity, causing immunosuppression and inflammation.

Key words： Litopenaeus vannamei mercuric chloride oxidative damage immunosuppression inflammation

Received: 12 January 2022

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	Articles by authors
	DONG Weifeng
	YANG Bicheng
	LI Guangyi
	DAI Hong
	LIU Zhiyong
	JIANG Hao

Cite this article:

DONG Weifeng,YANG Bicheng,LI Guangyi, et al. Acute Toxicity Assessment in Pacific White Shrimp Exposed to Different Concentrations of Mercuric Chloride[J]. Fisheries Science, 2024, 43(1): 97-103.

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https://www.shchkx.com/EN/10.16378/j.cnki.1003-1111.22007 OR https://www.shchkx.com/EN/Y2024/V43/I1/97

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