复方中草药制剂对克氏原螯虾抗白斑综合征感染研究

doi:10.16378/j.cnki.1003-1111.22043

Abstract
Figure/Table
References
Related Citation (15)

Download: PDF (1347 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract In order to evaluate the non-specific immunity, antioxidant capacity and anti-white spot syndrome virus (WSSV) infection effect of supplementation with compound Chinese herb medicine prepared byrhubarb root and rhizome, baical skullcap root, virgate wormwood herb, creeping euphorbia, largehead atractylodes rhizome, and isatis rootin red swamp crayfish Procambarus clarkiiin diet, juvenile red swamp crayfish with body weight of (8.94±1.16)g was reared in a 2 m×3 m×1 m aerated pond at stocking rate of 120 individuals per pond and fed the basic diet (control group) and supplemented with the compound Chinese herb medicine at a dose of 0.5%, 1.0%, 1.5% and 2.0% for 14 d. Then the non-specific immunity of hemolymph and the antioxidant capacity of hepatopancreas were determined, and the anti-infection ability of the red swamp crayfish against WSSV infection was analyzed. The results showed that the activities of lysozyme, phenol oxidase (PO), acid phosphatase (ACP) and alkaline phosphatase (AKP) in the serum of the red swamp crayfish fed the compound Chinese herb medicine were found to be increased by 4.07%-16.20% (P<0.05), 12.96%-31.94% (P<0.05), 32.36%-39.87% (P<0.05) and 36.50%-71.94% (P<0.05). The total superoxide dismutase (T-SOD), catalase (CAT) and total antioxidant capacity (T-AOC)in hepatopancreas were increased by 4.39%-25.76% (P<0.05), 32.85%-93.43% (P<0.05) and 21.52%-55.10% (P<0.05), and the malondialdehyde content in hepatopancreas was decreased by 26.22%-45.05% (P<0.05). The red swamp crayfish treated with compound Chinese herb medicine and then challenged with WSSV had increase insurvival rate by 13.33%-31.67% during the 5 d (P<0.05).The findings indicated that the addition of dietary compound Chinese herb medicine significantly enhanced the non-specific immunity, antioxidant capacity and resistance to WSSV infection in red swamp crayfish, with optimal dose of 1.0%.

Key words： compound Chinese herbs medicine preparation non-specific immunity antioxidant capacity white spot syndrome Procambarus clarkii

Received: 15 May 2022

S945.4

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	TIAN Haijun
	REN Shengjie
	YANG Zhiguo
	GU Xizhang

Cite this article:

TIAN Haijun,REN Shengjie,YANG Zhiguo, et al. Effect of Compound Chinese Herb Medicine on Resistance to White Spot Syndrome Infection in Red Swamp Crayfish Procambarus clarkii[J]. Fisheries Science, 2024, 43(1): 129-135.

URL:

https://www.shchkx.com/EN/10.16378/j.cnki.1003-1111.22043 OR https://www.shchkx.com/EN/Y2024/V43/I1/129

[1] 徐加涛, 阎斌伦, 徐国成. 克氏原螯虾产业发展背景、现状与展望[J]. 水产科技情报, 2011, 38(4):172-176.
[2] 何琦瑶, 汪开毓, 刘韬, 等. 湖北省潜江地区克氏原螯虾白斑综合征PCR诊断及组织病理学观察[J]. 水产学报, 2018, 42(1):131-140.
[3] 中华人民共和国农业农村部. 一二三类动物疫病病种目录(修订征求意见稿)[EB/OL]. http://www.moa.gov.cn/govpublic/xmsyj/202203/t20220308_6390994.htm.
[4] 邓平, 张立强, 艾桃山, 等. 3种中草药对克氏原螯虾肝胰腺抗氧化系统及组织结构的影响[J]. 江苏农业科学, 2020, 48(2):183-187.
[5] 孟愔, 胡骞, 金玉立, 等. 复方中草药制剂对克氏原螯虾生长、免疫功能及肝胰腺组织的影响[J]. 中国饲料, 2019(21):60-65.
[6] 孟愔. 复方中草药对克氏原螯虾生长、免疫功能及肝肠组织的影响[D]. 武汉: 华中农业大学, 2019.
[7] 郝忱, 万金娟, 沈美芳, 等. 中草药添加剂对克氏原螯虾生长及免疫力的影响[J]. 江苏农业学报, 2014, 30(2):363-369.
[8] 丁建英, 邵菁, 徐建荣,等. 复方中草药饲料添加剂对克氏原螯虾非特异性免疫功能的影响[J]. 安徽农业科学, 2012, 40(31):15266-15267.
[9] 国家药典委员会. 中华人民共和国药典:一部[M]. 北京: 中国医药科技出版社, 2020:155-168.
[10] 黄玉普, 吴大章, 王森. 黄芩的药理作用及其药对研究进展[J]. 中国药业, 2022, 31(15):129-133.
[11] 左军, 张金龙, 胡晓阳. 白术化学成分及现代药理作用研究进展[J]. 辽宁中医药大学学报, 2021, 23(10):6-9.
[12] 杨亚勤. 板蓝根药理作用及临床应用研究[J]. 中国继续医学教育, 2015, 7(3):252.
[13] 於叶兵, 刘飞, 王爱民, 等. 一种小龙虾中草药饲料添加剂, 制备方法及使用方法: CN112715784A[P]. 2021-04-30.
[14] 朱斐, 吕梦园, 许梓荣. 白斑综合征病毒感染克氏原螯虾后的PCR检测及组织病理学研究[J]. 淡水渔业, 2012, 42(6):43-48.
[15] 邹广众, 孙虎山. 水产甲壳动物免疫学研究进展与前景展望[J]. 生命科学仪器, 2009, 7(6):17-21.
[16] YIN X L, LI Z J, YANG K, et al. Effect of guava leaves on growth and the non-specific immune response of Penaeus monodon[J]. Fish & Shellfish Immunology, 2014, 40(1):190-196.
[17] WEI J, YU N, TIAN W J, et al. Dietary vitamin B₁₂ requirement and its effect on non-specific immunity and disease resistance in juvenile uvenile Chinese mitten crab Eriocheir sinensis[J]. Aquaculture, 2014, 434:179-183.
[18] LIU B, GE X P, XIE J, et al. Effects of anthraquinone extract from Rheum officinale Bail on the physiological responses and HSP70 gene expression of Megalobrama amblycephala under Aeromonas hydrophila infection[J]. Fish & Shellfish Immunology, 2012, 32(1):1-7.
[19] ZHOU L J, LIU Z J, WANG Z X, et al. Astragalus polysaccharides exerts immunomodulatory effects via TLR4-mediated MyD88-dependent signaling pathway in vitro and in vivo[J]. Scientific Reports, 2017,7:44822.
[20] ZHAO T, FENG Y, LI J, et al. Schisandra polysaccharide evokes immunomodulatory activity through TLR4-mediated activation of macrophages[J]. International Journal of Biological Macromolecules, 2014, 65:33-40.
[21] LIU L, YUAN S F, LONG Y, et al. Immunomodulation of Rheum tanguticum polysaccharide (RTP) on the immunosuppressive effects of dexamethasone(DEX) on the treatment of colitis in rats induced by 2, 4, 6-trinitrobenzene sulfonic acid[J]. International Immunopharmacology, 2009, 9(13/14):1568-1577.
[22] GONG A G, ZHANG L M, LAM C T, et al. Polysaccharide of Danggui Buxue Tang,an ancient Chinese herbal decoction,induces expression of pro-inflammatory cytokines possibly via activation of NFκB signaling in cultured RAW 264.7 cells[J]. Phytotherapy Research, 2017, 31(2):274-283.
[23] JIN B, SUN T, YU X H, et al. The effects of TLR activation on T-cell development and differentiation[J]. Clinical & Developmental Immunology, 2012, 2012:836485.
[24] 段亚飞, 董宏标, 王芸, 等. 干露胁迫对日本囊对虾抗氧化酶活性的影响[J]. 南方水产科学, 2015, 11(4):102-108.
[25] 刘波, 明俊超, 谢骏, 等. 大黄蒽醌提取物对罗氏沼虾高温下抗氧化能力与热应激蛋白70基因表达的影响[J]. 水产学报, 2010, 34(6):972-980.
[26] WEI K Q, YANG J X. Histological alterations and immune response in the crafish Procambarus clarkii given rVP28-incorporated diets[J]. Fish & Shellfish Immunology, 2011, 31(6):1122-1128.
[27] 刘永涛, 董靖, 夏京津, 等. 不同饲料对稻田养殖克氏原螯虾生长、非特异性免疫酶及体成分的影响[J]. 中国渔业质量与标准, 2020, 10(1):43-51.
[28] 任秀芳, 柏爱旭, 张萍, 等. 壳聚糖对水质和克氏原螯虾仔虾非特异性免疫酶的影响[J].江苏农业科学, 2013, 41(9):202-204.
[29] 王海锋, 成永旭, 李京昊, 等. 干露和再入水对克氏原螯虾抗氧化应激能力的影响[J].南方水产科学, 2019, 15(5):69-76.
[30] 杨维维, 沈美芳, 刘文斌, 等. 大黄素对克氏螯虾生长、免疫、肝脏抗氧化以及肠道消化酶的影响[J]. 江苏农业学报, 2013, 29(6):1405-1410.
[31] 冯具攀. 谷胱甘肽、黄芪多糖和维生素C对克氏原螯虾抗高温应激能力及生长的影响[D].上海: 上海海洋大学, 2021.
[32] 陈辉辉, 涂晨凌, 唐杨, 等. 复方中草药对白斑综合征病毒感染下凡纳滨对虾免疫活性的影响[J]. 厦门大学学报(自然科学版), 2017, 56(5):686-692.
[33] 景亚运. HD-3复方中草药对凡纳滨对虾免疫酶影响和抗WSSV感染分析[D]. 大连: 大连海洋大学, 2016.
[34] 叶建生, 阎斌伦, 王兴强. 中草药制剂对凡纳滨对虾非特异性免疫的影响[J]. 扬州大学学报(农业与生命科学版), 2018, 39(1):25-30.
[35] 黄忠, 林黑着, 李卓佳, 等. 复方中草药投喂策略对凡纳滨对虾生长、消化及非特异性免疫功能的影响[J]. 南方水产科学, 2013, 9(5):37-43.
[36] 王芸, 李健, 陈萍, 等. 复方中草药对凡纳滨对虾生长及非特异性免疫功能的影响[J]. 中国农学通报, 2012, 28(29):109-114.