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| Effects of Florfenicol Residues on Growth and Antioxidant System of Chlorella sp. |
| WANG Baolong1, ZHANG Tao1,2, ZHANG Weixiao1,2, HUANG Ruipin1, MA Pengfei1,2, LI Na1,2, ZHANG Yingqi1, XUE Mingyu2, ZHANG Qian1,2, LIU Ying1 |
1. Key Laboratory of Environment Controlled Aquaculture, Ministry of Education, Dalian Ocean University, Dalian 116023, China;
2. College of Marine Science and Environment, Dalian Ocean University, Dalian 116023, China |
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Abstract With the intensive development of aquaculture, antibiotic residues in aquaculture effluent posed a serious threat to aquatic ecosystem, which has aroused global attention. In present study, the commonly used diet microalga Chlorella sp. in aquaculture was taken as the research object to explore the effects of florfenicol on marine microalgae. The effects of florfenicol exposure at different concentrations for 72 h on the growth, the contents of pigments, reactive oxygen species (ROS), malondialdehyde (MDA) content and antioxidant enzyme activities of Chlorella sp. were analyzed. The results showed that florfenicol at low concentration promoted the growth of Chlorella sp., while inhibited the growth of Chlorella sp. at high concentration, and the contents of chlorophyll varied in a reverse U-shaped trend with the increase in mass concentration of florfenicol, with the maximal chlorophyll content in 0.100 mg/L florfenicol group, which was 74.6% higher than that in control group. Florfenicol exposure may cause the oxidative damage to the cell membrane of microalgae, 20 mg/L florfenicol led to the increase of MDA contents and stimulates the carotenoids synthesis, was 2.45 times higher than that in the control group, which may participate in the resistance to oxidative damage. Under the experimental concentrations, the activities of glutathione (GSH), superoxide dismutase (SOD) and catalase (CAT) were significantly inhibited. Compared to Isochrysis galbana, Chlorella sp. has a high tolerance to florfenicol. The finding provides theoretical basis for the treatment of antibiotic residues in aquaculture effluent.
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Received: 01 June 2022
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