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| Effects of Different Diets on Metamorphosis of Megalopa in Mud Crab Scylla paramamosain |
| QIAO Guangde1,2, MA Chunyan2, ZHANG Fengying2, WANG Wei2, ZHAO Ming2, CHEN Wei2, FU Yin1,2 , LIU Zhiqiang2, MA Lingbo2 |
1. College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China;
2. Genetic Breeding and Biotechnology Laboratory, East China Sea Fishery Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, China |
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Abstract In order to find the suitable diets for megalopa of mud crab Scylla paramamosain during its metamorphosis period, (1000±30) megalopa larvae were reared in an incubation bucket containing 360 L water and fed Artemia nauplius, frozen copepods, frozen adult Artemia, formulated feed and no feeding until the metamorphosed into crabs to evaluate effects of feeding different diets on the water quality in aquaculture water, the molting metamorphosis of the larvae, the activity of immune-related enzymes and digestive enzymes. The results showed that there were the minimal ammonia nitrogen content and nitrite content and the maximal survival rate in the cultured water in Artemia nauplius group. The megalopa larvae had 1—3 days shorter development period in the Artemia nauplii group than that in the other three groups, with significantly longer carapace length and carapace width than those in the formulated diets group, without significant difference between frozen copepods group and frozen Artemia groups. There were no significant differences in acid phosphatase activity, alkaline phosphatase activity, superoxide dismutase activity and α-amylase activity among the four groups. The lysozyme activity and total antioxidant capacity were shown to be not significantly different in the Artemia nauplius group from those in frozen copepods and frozen Artemia groups, significantly higher than those in the formulated diet group. There were significantly higher trypsin and lipase activities in Artemia group than those in the other three groups, indicating that the Artemia nauplii were more suitable as a diet for the megalopa of mud crab, which positively affected the water quality, the molting survival rate, immunoenzyme activity and digestive activity of the juvenile crab. The effects of frozen copepods and frozen Artemia were both better than formulated diets, though they had slightly worse effect. It was found that frozen copepods and frozen Artemia were used as partial substitutes for Artemia nauplii during the larval rearing of the mud crab megalopa, which can help to reduce the production costs, simplify the production process, and eventually promote the large-scale production of seedlings in this crab. The findings provided the basis for the selection of food during the artificial cultivation of the mud crab seed.
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Received: 07 December 2022
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