低氧胁迫对四角蛤蜊存活、抗氧化指标和呼吸相关酶的影响

doi:10.16378/j.cnki.1003-1111.23026

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Abstract In order to probe the effects of hypoxic stress on the survival, antioxidant indices and respiration-related enzyme activities of surf clam Mactra veneriformis, the surf clam with shell length of (35.91±2.31) mm were put into a plastic box and exposed to dissolved oxygen concentrations of (2.0±0.2) mg/L and (4.0±0.2) mg/L by continuous nitrogen filling, and to dissolved oxygen concentration of (7.5±0.2) mg/L by continuous oxygenation (control group) at water temperature of (20±1) ℃. The survival, antioxidant indices (superoxide dismutase, catalase and malondialdehyde) and activities of respiration-related enzymes (lactate dehydrogenase and pyruvate kinase) were measured at 0, 6, 12, 24, 48 and 72 h of hypoxia stress. The results showed that the survival rate of surf clam was decreased with the decrease in dissolved oxygen concentration, and the stress period, with survival rate of 100% during the experiment in the control group, and dead individuals in 36 h in the treatment groups. There were survival rates of 97.5% in 48 hours in 4.0 mg/L group and 91.67% in 2.0 mg/L group , and at 72 hours the survival rates of 78.66% in 4.0 mg/L group and 67.30% in 2.0 mg/L group. The enzyme activities were significantly affected by stress time and dissolved oxygen concentration (P<0.05), and primarily stable during the experimental period in the control group. An overall trend of first increase and then decrease in antioxidant indices and activities of respiratory related enzymes was observed in 4.0 mg/L group and 2.0 mg/L group, with the maximal activities of superoxide dismutase and catalase in hepatopancreas at 6 h, significantly higher than those at other time points. The activity of pyruvate kinase in the gill filaments was found to be higher in the treatment groups, with the maximum content of malondialdehyde at 12 hours, than that in the control group. The findings will help to clarify the mechanism of the effects of hypoxia stress on the physiological and biochemical activities of surf clam, and provide a reference with further exploring the mechanism of hypoxia tolerance of surf clam and the creation of new germplasm resistant to hypoxia.

Key words： Mactra veneriformis hypoxic stress survival rate antioxidant index respiratory related enzyme

Received: 13 April 2023

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	Articles by authors
	YU Shuai
	SONG Yilin
	CHEN Limei
	LIANG Jian
	GUO Yongjun

Cite this article:

YU Shuai,SONG Yilin,CHEN Limei, et al. Effects of Hypoxia Stress on Survival, Antioxidant Indices and Activities of Respiration-Related Enzymes of Surf Clam Mactra veneriformis[J]. Fisheries Science, 2024, 43(4): 606-613.

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https://www.shchkx.com/EN/10.16378/j.cnki.1003-1111.23026 OR https://www.shchkx.com/EN/Y2024/V43/I4/606

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