响应面法优化大孔树脂纯化仿刺参中皂苷工艺研究

doi:10.16378/j.cnki.1003-1111.23080

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Abstract In order to optimize the optimum process conditions for purifying saponin Holotoxin A₁ (HA₁) by macroporous resin, four different structural macroporous resins (AB-8, HP-20, D101 and NKA-9) commonly used for purification of saponins in sea cucumber Apostichopus japonicus were selected. The content of HA₁ was determined by high performance liquid chromatography (HPLC) using sea cucumber saponin HA₁ as a standard. By comparing their static adsorption-desorption capacity and static adsorption curves, the suitable resin type was selected. The crude saponin mass concentration, ethanol volume fraction and ethanol washing volume were used as factors to determine the optimal dynamic purification process conditions based on the single factor experiments and Box-Behnken response surface experiment. The results showed that AB-8 resin had the best purification effect on HA₁, and the optimal purification process was as follows: crude saponin mass concentration of 25 mg/mL, flow rate 1 mL/min, sample loading volume 25 mL, washing volume 2 times column volume (BV), ethanol volume fraction 73%, and ethanol washing volume 7 BV. Under these conditions, the recovery rate of HA₁ was 91.40%, and the purity was increased from 0.16% to 0.58%. The purification process obtained in this study is suitable for the purification of saponin HA₁ in Apostichopus japonicus.

Key words： Apostichopus japonicus saponin Holotoxin A₁ macroporous resin response surface methodology

Received: 09 October 2023

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	LIU Yujun
	LIU Guiying
	YU Di
	LI Long
	FU Zhiyu
	ZHENG Jie
	LIU Yutong
	ZHOU Zunchun

Cite this article:

LIU Yujun,LIU Guiying,YU Di, et al. Optimization of Purification Process of Saponins from Sea Cucumber Apostichopus japonicus with Macroporous Resin by Response Surface Methodology[J]. Fisheries Science, 2024, 43(5): 727-736.

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https://www.shchkx.com/EN/10.16378/j.cnki.1003-1111.23080 OR https://www.shchkx.com/EN/Y2024/V43/I5/727

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