凹凸棒石对锦鲤生长、消化及其肠道菌群的影响

doi:10.16378/j.cnki.1003-1111.23096

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Abstract In order to investigate the effects of different attapulgite additions on the growth performance, digestive enzyme activities, and intestinal flora and histological structure of koi carp Cyprinus carpio, 240 healthy and disease-free koi carp with body weight of (115.48±3.72) g were randomly divided into 4 groups with 3 replicates in each group and 20 fish in each replicate,and fed the basic diet containing 0% (control group), 3%, 5% and 8% of attapulgite for 28 days. The results showed that dietary attapulgite led to increase the activities of intestinal α-amylase, lipase and trypsin, with significant effect at 0% and 3% groups(P<0.05), even though without significant effect on the growth performance of koi carp (P>0.05). There were increase in the intestinal mucosal fold height and muscle thickness in the test groups compared with the control group, with significant effect at 3% and 5% groups (P<0.05). There were sequencing coverage of 99.8% in samples in each group, and the gradually gentle dilution curve, indicating that the sequencing results were reasonable and reliable. At the phylum level, the dominant bacteria were found to be Firmicutes, Proteobacteria, and Fusobacteria in each group, with the dominant bacteria of Cetobacterium, Bacillus, and Peptostreptococcus at the genus level. At different classification levels, the higher similarity of community composition was observed in the 0% and 3% groups, and the analysis showed that attapulgite promotes the reproduction of beneficial bacteria and improves the composition and distribution of intestinal flora.

Key words： Cyprinus carpio attapulgite growth performance digestive enzyme intestinal histological structure intestinal flora

Received: 01 December 2023

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	Articles by authors
	YANG Yuanyuan
	TU Dongyu
	YU Hong
	FANG Zhenzhen
	SHI Hongyue
	SUN Xueliang
	CHEN Chengxun

Cite this article:

YANG Yuanyuan,TU Dongyu,YU Hong, et al. Effects of Attapulgite on Growth, Digestion and Intestinal Flora of Koi Carp Cyprinus carpio[J]. Fisheries Science, 2024, 43(6): 955-964.

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https://www.shchkx.com/EN/10.16378/j.cnki.1003-1111.23096 OR https://www.shchkx.com/EN/Y2024/V43/I6/955

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