|
|
|
| Effect of Temperature Acclimation on Growth and Thermal Tolerance of Juvenile Rhynchocypris lagowskii |
| BO Qikang1, BAI Xiaohui1, WANG Xiaoyu1, LIU Keming1, HE Xiaoxu1, PEI Yue2, ZHANG Wei1 |
1. Tianjin Fisheries Research Institute, Tianjin 300221, China;
2. Tianxiang Aquaculture Co., Ltd. of Tianjin, Tianjin 301500, China |
|
|
|
|
Abstract In order to investigate effects of different acclimation temperatures on the growth and temperature tolerance of juvenile Rhynchocypris lagowskii, juveniles with initial body weight of (7.96±1.84) g were reared in recirculating water tanks of each 1.0 m×0.6 m×0.8 m with water depth of 0.6 m at stocking density of 40 fish per tank at acclimation temperatures of 20, 25 and 28 ℃ to probe into the effects of acclimation temperature on growth, liver and foregut digestive enzymes and transaminase activities for 33 d. At the same time, the juveniles were reared in 10 L barrels with a diameter of 25 cm and a water depth of 20 cm and acclimated at 15, 20, 25 and 28 ℃ to find out the critical temperature, acclimation response rate and temperature tolerance range. After the juveniles were stabilized, warming/cooling was begun at a constant rate of 1.0 ℃/h. When the fish body was initially out of equilibrium, the temperature was the maximum critical temperature (TCmax) and the minimum critical temperature (TCmin) (accuracy of 0.1)], and the fish was released to room temperature for recovery. The results showed that the juveniles grew normally at water temperature of 20 and 25 ℃ during the culture, death was initially observed at water temperature of 28 ℃ with a survival rate of 86.67%. There were significantly increase in final body weight [(9.13±2.22) g and (10.01±2.74) g, respectively], body length [(8.64±0.73) cm and (8.89±0.82) cm]and total length [(10.18±0.79) cm and (10.49±0.95) cm] in the juveniles in 20 ℃ and 25 ℃ groups compared with the initial growth parameters (P<0.05), without significant difference in 28 ℃ group (P>0.05). The activities of amylase and lipase were higher in the liver and intestines of juveniles in the 25 ℃ treatment group, without significantly higher activity of hepatic amylase in 28 ℃ group, without significant difference in liver and intestinal protease activities among the three domesticated groups of juvenile fish(P>0.05). The activity of intestinal amylase was shown to be significantly higher in the 25 ℃ treatment group, with significantly higher activity of hepatic lipase than that in the other two groups and significantly higher activity of intestinal lipase than that in 20 ℃ group(P<0.05). No significant difference in activity of glutamic-pyruvic transaminase (ALT) was observed between 20 ℃ group and 25 ℃ group (P>0.05), with the temperature increasing to 25 ℃, significant decrease in activity of ALT(P<0.05). The activity of glutamic oxalacetic transaminase (AST) was found to be significantly decreased first and then significantly increased with the increasing temperature, with significant higher level in 28 ℃ group than that in other two groups (P<0.05), indicating that the long-term high temperature induced damage to the liver of juvenile fish, which was the cause of death of juvenile fish. Temperature acclimation affected the thermal tolerance of the juvenile fish, and high temperature led to increase the maximum critical temperature and the minimum critical temperature, with the maximum high-temperature acclimation response ratio of (0.86) within 25—28 ℃, and the maximum low-temperature acclimation response ratio value of (0.60) within 20—25 ℃ as well as the thermal tolerance polygon of 403.77 ℃2. In conclusion, like the temperature tolerance of tropical fish, R. lagowskii has strong temperature adaptation ability and can potentially compensate for the negative effects of temperature rise or fall by adjusting physiological and biochemical processes under near-extreme temperature conditions.
|
|
Received: 30 January 2024
|
|
|
|
|
|
[1] 吴加莹,戴明姝,刘志刚,等.温度对织锦巴非蛤稚贝生存和生长的影响[J].南方水产科学,2023,19(2):62-69.
[2] 高权新,谢明媚,彭士明,等.急性温度胁迫对银鲳幼鱼代谢酶、离子酶活性及血清离子浓度的影响[J].南方水产科学,2016,12(2):59-66.
[3] BEITINGER T L, BENNETT W A, MCCAULEY R W. Temperature tolerances of North American freshwater fishes exposed to dynamic changes in temperature[J]. Environmental Biology of Fishes,2000,58(3):237-275.
[4] 任中华,宋骏杰,刘永叶,等.辽东湾4种海洋生物的热耐受性研究[J].渔业科学进展,2018,39(3):1-10.
[5] REYNOLDS W W. Temperature as a proximate factor in orientation behavior[J]. Journal of the Fisheries Research Board of Canada,1977,34(5):734-739.
[6] CAMPOS D F, AMANAJÁS R D, ALMEIDA-VAL V M F, et al. Climate vulnerability of South American freshwater fish:thermal tolerance and acclimation[J]. Journal of Experimental Zoology. Part A: Ecological and Integrative Physiology,2021,335(9/10):723-734.
[7] 胡彦波,李昀,温海深,等.不同群体花鲈幼鱼温度耐受特征的初步研究[J].中国海洋大学学报(自然科学版),2019,49(增刊Ⅱ):1-7.
[8] 俞丹,沈中源,张智,等.温度驯化对尖头鳉热耐受特征的影响[J].水生生物学报,2017,41(3):538-542.
[9] COOPER C J, MUELLER C A, EME J. Temperature tolerance and oxygen consumption of two South American tetras, Paracheirodon innessi and Hyphessobrycon herbertaxelrodi[J]. Journal of Thermal Biology,2019,86:102434.
[10] 谢鹏.中国鱥属鱼类形态学比较和温度适应的研究[D].武汉:华中农业大学,2019.
[11] 刘艳辉,陈伟强,刘铁钢,等.拉氏鱥的养殖现状及发展前景[J].水产科技情报,2015,42(5):255-257.
[12] 骆小年,李军,金广海,等.拉氏鱥人工繁殖试验[J].水产科学,2013,32(11):673-675.
[13] 任星月,骆小年,邓舒萍,等.拉氏鱥仔稚鱼发育、饥饿不可逆点及适时下塘研究[J].水产科学,2023,42(4):594-603.
[14] 吴晨,骆小年,段友健,等.不同离子浓度、温度、pH对拉氏鱥精子活力的影响[J].水产科学,2022,41(2):210-217.
[15] 骆小年,李军,杨培民,等.拉氏鱥池塘苗种培育试验[J].水产科学,2014,33(3):186-189.
[16] 刘艳辉,祖岫杰,刘铁钢,等.野生及养殖拉氏鱥营养成分比较分析[J].吉林农业大学学报,2017,39(1):82-88.
[17] 张帅.拉氏鱥染色体核型及D-loop遗传多样性分析[D].大连:大连海洋大学,2016.
[18] 杨培民,胡宗云,金广海,等.用线粒体D-loop和Cyt b基因序列分析拉氏鱥3个群体的遗传结构和遗传分化[J].水产学杂志,2017,30(4):7-12.
[19] CAMPOS D F, JESUS T F, KOCHHANN D, et al. Metabolic rate and thermal tolerance in two congeneric Amazon fishes:Paracheirodon axelrodi Schultz,1956 and Paracheirodon simulans Géry,1963 (Characidae)[J]. Hydrobiologia,2017,789(1):133-142.
[20] 付监贵,张振早,李彩娟,等.温度对不同体质量梭鲈幼鱼耗氧率、排氨率及窒息点的影响[J].水产养殖,2018,39(10):18-22.
[21] 邵彦翔.石斑鱼对温度胁迫的耐受性研究[D].大连:大连海洋大学,2016.
[22] 刘克明,钱红,夏苏东,等.温度对北极红点鲑体成分和消化酶活力指标的影响[J].经济动物学报,2017,21(1):29-32.
[23] 刘鉴毅,宋志明,王妤,等.温度对点篮子鱼幼鱼生长、摄食和消化酶活性的影响[J].海洋渔业,2015,37(5):442-448.
[24] 杨超超,谭婷,杨娜,等.菲胁迫对泥鳅转氨酶活性及肝脏组织结构的影响[J].生态学杂志,2023,42(6):1443-1448.
[25] UNER N, ORUÇ E O, CANLI M, et al. Effects of cypermethrin on antioxidant enzyme activities and lipid peroxidation in liver and kidney of the freshwater fish, Oreochromis niloticus and Cyprinus carpio (L. )[J]. Bulletin of Environmental Contamination and Toxicology,2001,67(5):657-664.
[26] CURRIE R J, BENNETT W A, BEITINGER T L. Critical thermal minima and maxima of three freshwater game-fish species acclimated to constant temperatures[J]. Environmental Biology of Fishes,1998,51(2):187-200.
[27] 雷忻,钟瑞,刘利娅,等.对硝基苯酚对泥鳅肝组织结构及丙氨酸转氨酶活性的影响[J].西北农林科技大学学报(自然科学版),2013,41(9):15-20.
[28] 付康康,曹振东,付世建.温度驯化对高体鳑鲏热耐受及低氧耐受能力的影响[J].生态学杂志,2015,34(6):1586-1590.
[29] 崔雯婷,宋骏杰,田洪林,等.黄茅海五种常见海洋生物的热耐受性[J].水产学报,2018,42(4):522-533.
[30] 窦硕增,南鸥,曹亮,等.石岛湾四种常见鱼类的热耐受性比较研究[J].海洋科学,2017,41(9):56-64.
[31] OSPINA A F, MORA C. Effect of body size on reef fish tolerance to extreme low and high temperatures[J]. Environmental Biology of Fishes,2004,70(4):339-343.
[32] 蔡泽平,陈浩如,金启增,等.热废水对大亚湾三种经济鱼类热效应的研究[J].热带海洋,1999,18(2):11-19.
[33] 王云松.南方鲇、瓦氏黄颡鱼和中华倒刺鲃幼鱼的热耐受特征及其比较研究[D].重庆:重庆师范大学,2009.
[34] 陈全震,曾江宁,高爱根,等.鱼类热忍耐温度研究进展[J].水产学报,2004,28(5):562-567.
[35] 刘丽丽,朱华,闫艳春,等.鱼类低温耐受机制与功能基因研究进展[J].生物技术通报,2018,34(8):50-57.
[36] YANAR M, ERDOGˇAN E, KUMLU M. Thermal tolerance of thirteen popular ornamental fish species[J]. Aquaculture,2019,501:382-386.
[37] CHATTERJEE N, PAL A K, MANUSH S M, et al. Thermal tolerance and oxygen consumption of Labeo rohita and Cyprinus carpio early fingerlings acclimated to three different temperatures[J]. Journal of Thermal Biology,2004,29(6):265-270. |
|
|
|
