慢性氨氮胁迫对团头鲂生长、肠道酶活性及结构的影响

doi:10.16378/j.cnki.1003-1111.24107

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Abstract In order to investigate the effects of chronic ammonia nitrogen stress on growth, and intestinal enzymes activity and histological structure of blunt snout bream, Megalobrama amblycephala, blunt snout bream with body weight of (6.55±0.24) g was reared in a 120 cm height × 80 cm diameter tank, and exposed to aerated tap water (control group G0), 6.82 mg/L ammonium chloride (G1) (10% 96 h LC₅₀) and 13.64 mg/L ammonium chloride (G2) (20% 96 h LC₅₀) at 26—27 ℃ for 4 weeks. At the end of the experiment, enzyme activity analysis, transmission micro-electron microscopy and PCR technology were conducted to analyze the intestinal enzyme activity and histological structure characteristics of the test fish. The results showed that the median lethal concentration LC₅₀ of ammonia nitrogen solution at 96 h was 68.18 mg/L for blunt snout bream. The final body weight, survival rate, weight gain rate, specific growth rate, and activities of protease, lipase, amylase, Na⁺/K⁺-ATPase, and γ-GT were shown to be significantly higher in the fish in G0 group than those in G1 and G2 groups (P<0.05). There was significantly higher intestinal microvillus length in blunt snout bream in G0 than those in G1 and G2 groups (P<0.05). Chronic ammonia nitrogen stress led to down-regulated relative expression levels of Claudin-3c, Claudin-7, Occludin and ZO-1 genes compared to those in G0 group, and to up-regulated relative expression levels of TNF-a, IL-1B and IL-6 (P<0.05). In conclusion, chronic ammonia nitrogen stress inhibited the growth, and decreased digestive and absorptive function of blunt snout bream.

Key words： chronic ammonia nitrogen stress growth intestine enzyme activity tight junction protein blunt snout bream

Received: 19 June 2024

PACS:

S965.119

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	Articles by authors
	WANG Canli
	DAI Mengyang
	WANG Qian
	XIONG Xinyu
	ZHANG Han
	WANG Yifan
	YE Wenhui
	WANG Hengjie
	YUAN Xiangyang

Cite this article:

WANG Canli,DAI Mengyang,WANG Qian, et al. Effects of Chronic Ammonia Nitrogen Stress on Growth, Intestinal Digestive Enzyme Activity and Structure of Blunt Snout Bream Megalobrama amblycephala[J]. Fisheries Science, 2025, 44(4): 573-581.

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https://www.shchkx.com/EN/10.16378/j.cnki.1003-1111.24107 OR https://www.shchkx.com/EN/Y2025/V44/I4/573

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