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| Seasonal Changes in Fish Community in Jinjiang River Based on Environmental DNA Metabarcoding |
| LI Sihui1, DENG Weide2, ZOU Lu3, SUN Zhenzhu2, LI Yuan2, XU Youhou1, WEN Jing2,4 |
1. College of Marine Science, Beibu Gulf University, Qinzhou 535011, China;
2. School of Biology and Agriculture, Shaoguan University, Shaoguan 512005, China;
3. Xining Customs Technical Center, Qinghai, Xining 810000, China;
4. Guangdong Provincial Key Laboratory of Utilization and Conservation of Food and Medicinal Resources in Northern Region, Shaoguan University, Shaoguan 512005, China |
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Abstract Jinjiang River is the main branch of the Pearl River system. The Jinjiang River flows through Danxia, which is honored with Global Geoparks, World Heritage, China National Park, National Nature Reserve and National AAAAA Tourist Attractions.In order to understand the fish species diversity of Jinjiang River and Danxiashan in the Pearl River system, mixed water samples were collected from surface and bottom at 15 survey stations in Jinjiang River as the main tributary of the Pearl River system in November 2023, February, May and August 2024, and the changes in fish community in the four seasons were monitored by eDNA macro barcodes. The results showed that 2 622 108 reads, 99 fish species, were obtained for fish from 180 samples of 15 survey stations after bioinformatics filtering, which belong to 12 orders 21 families 69 genera, without significant difference in α diversity index among the different types of waters between Danxiashan and Non-Danxiashan. There was significant difference in fish community structure between spring and autumn, and no significant difference among other seasons. Seven invasive alien species were found, especially large number of Coptodon zillii in all survey stations in four seasons. The second-class national protected animal Hemibagrus guttatus was observed in 10 survey stations in four seasons. The findings provide detailed data on fish diversity and important implications for the long-term management and conservation of the Jinjiang river, and prove that eDNA has great potential in the research of fish diversity in river systems.
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Received: 17 February 2025
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[1] WANG X Y, LU G Q, ZHAO L L, et al. Assessment of fishery resources using environmental DNA:the large yellow croaker (Larimichthys crocea) in the East China Sea[J]. Fisheries Research, 2021,235:105813.
[2] HE W L, XU D P, LIANG Y D, et al. Using eDNA to assess the fish diversity and spatial characteristics in the Changjiang River-Shijiu Lake connected system[J]. Ecological Indicators,2022,139:108968.
[3] HARPER L R, LAWSON HANDLEY L, SAYER C D, et al. Assessing the impact of the threatened crucian carp (Carassius carassius) on pond invertebrate diversity:a comparison of conventional and molecular tools[J]. Molecular Ecology,2021,30(13):3252-3269.
[4] STOECKLE M Y, ADOLF J, CHARLOP-POWERS Z, et al. Trawl and eDNA assessment of marine fish diversity, seasonality, and relative abundance in coastal New Jersey, USA[J]. ICES Journal of Marine Science,2021,78(1):293-304.
[5] ZOU K S, CHEN J W, RUAN H T, et al. eDNA metabarcoding as a promising conservation tool for monitoring fish diversity in a coastal wetland of the Pearl River Estuary compared to bottom trawling[J]. Science of the Total Environment,2020,702:134704.
[6] STRAYER D L. Alien species in fresh waters:ecological effects, interactions with other stressors, and prospects for the future[J]. Freshwater Biology,2010,55:152-174.
[7] MIYA M, SATO Y, FUKUNAGA T, et al. MiFish, a set of universal PCR primers for metabarcoding environmental DNA from fishes:detection of more than 230 subtropical marine species[J]. Royal Society Open Science,2015,2(7):150088.
[8] EDGAR R C. UPARSE:highly accurate OTU sequences from microbial amplicon reads[J]. Nature Methods,2013,10(10):996-998.
[9] SCHNELL I B, BOHMANN K. Tag jumps illuminated-reducing sequence-to-sample misidentifications in metabarcoding studies[J]. Molecular Ecology Resources,2015,15(6):1289-1303.
[10] LUO S, WANG M, MA W Z, et al. Environment DNA reveals fish diversity in a canyon river within the upper Pearl River drainage[J]. Animals,2024,14(16):2433.
[11] 朱书礼,陈蔚涛,武智,等.基于环境DNA技术的珠江中下游鱼类多样性初步研究[J]. 南方水产科学,2024,20(1):120-129.
[12] 许佳,崔小娟,张翼飞,等.南岭地区鱼类多样性及其地理分布[J]. 生物多样性,2024,32(7):159-169.
[13] XIE R L, ZHAO G F, YANG J H, et al. eDNA metabarcoding revealed differential structures of aquatic communities in a dynamic freshwater ecosystem shaped by habitat heterogeneity[J]. Environmental Research,2021,201:111602.
[14] PUKK L, KANEFSKY J, HEATHMAN A L, et al. eDNA metabarcoding in lakes to quantify influences of landscape features and human activity on aquatic invasive species prevalence and fish community diversity[J]. Diversity and Distributions,2021,27(10):2016-2031.
[15] 赵文玉,刘梦月,林本平,等. 基于eDNA技术的湘江长沙段丰水期鱼类物种多样性研究[J]. 水生态学杂志,2025,46(2):134-143.
[16] 王丹丹,李欣,宋洁,等.基于环境DNA的夏季鸭绿江流域(丹东段)鱼类多样性分析[J]. 南方农业学报,2024,55(6):1549-1560.
[17] MILHAU T,VALENTINI A, POULET N, et al. Seasonal dynamics of riverine fish communities using eDNA[J]. Journal of Fish Biology,2021,98(2):387-398.
[18] KIMBERG P, WOODFORD D, ROUX H, et al. Species-specific impact of introduced largemouth bass Micropterus salmoides in the Groot Marico Freshwater Ecosystem Priority Area, South Africa[J]. African Journal of Aquatic Science,2014,39(4):451-458.
[19] 沈梅,郭宁宁,罗遵兰,等.基于eDNA metabarcoding探究北京市主要河流鱼类分布及影响因素[J]. 生物多样性,2022,30(7):134-145.
[20] LI Z Y, JIANG P W, WANG L X, et al. A comparison of seasonal composition and structure of fish community between environmental DNA technology and gillnetting in the Pearl River Estuary, China[J]. Ecological Indicators,2023,147:109915.
[21] 董智玲,陈莎莎,吕宏森,等.基于环境DNA技术的长江上游重庆市江北段鱼类多样性研究[J]. 生态毒理学报,2023,18(6):1-15.
[22] 张彦彦,唐文乔,陈振锋,等.基于eDNA宏条形码技术的上海骨干河流鱼类多样性研究[J]. 长江流域资源与环境,2023,32(7):1433-1446.
[23] XU M, LI S P, LIU C L, et al. Global freshwater fish invasion linked to the presence of closely related species[J]. Nature Communications,2024,15(1):1411.
[24] GU D E, HU Y C, XU M, et al. Fish invasion in the river systems of Guangdong Province, South China:possible indicators of their success[J]. Fisheries Management and Ecology,2018,25(1):44-53.
[25] GRAMMER G, SLACK W, PETERSON M, et al. Nile tilapia Oreochromis niloticus (Linnaeus, 1758) establishment in temperate Mississippi, USA:multi-year survival confirmed by otolith ages[J]. Aquatic Invasions,2012,7(3):367-376.
[26] 农业农村部渔业渔政管理局,全国水产技术推广总站,中国水产学会.2024中国渔业统计年鉴[M]. 北京:中国农业出版社,2024.
[27] ZHOU A G, TANG H J, ZHANG L, et al. Dynamics of bacterial community and antibiotic resistance genes in the aquaculture ponds of channel catfish (Ietalurus punetaus):an association with the mobile genetic elements and environmental factors[J]. Aquaculture,2023,562:738726.
[28] LIU L H, YU L J, FU X Z, et al. First report of megalocytivirus (Iridoviridae) in cultured bluegill sunfish, Lepomis macrochirus, in China[J]. Microbial Pathogenesis,2019,135:103617.
[29] QIN Y H, ZHANG P P, ZHANG M F, et al. Isolation and identification of a new strain Micropterus salmoides rhabdovirus (MSRV) from largemouth bass Micropterus salmoides in China[J]. Aquaculture,2023,572:739538.
[30] XIE Y, LI Z Y, GREGG W P, et al. Invasive species in China—an overview[J]. Biodiversity & Conservation,2001,10(8):1317-1341.
[31] LIANG Y, LIU H Z, ZHAI S W, et al. Effects of weaning American glass eels (Anguilla rostrata) with the formula diet on intestinal microbiota and inflammatory cytokines genes expression[J]. Heliyon,2023,9(6):e16965.
[32] CHEN W T, XIANG D G, GAO S, et al. Whole-genome resequencing confirms the genetic effects of dams on an endangered fish Hemibagrus guttatus (Siluriformes:Bagridae):a case study in a tributary of the Pearl River[J]. Gene,2024,895:148000. |
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