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| High-Efficiency Reproductive Regulation Technology for Fat Minnow Rhynchocypris lagowskii |
| JIAN Zicong1,2, LUO Xiaonian1,2, DUAN Youjian1,2, LI Jiao1,2, DONG Ruixin1,2, LU Naida3, MENG Qingli3, JIN Guanghai4, YANG Peimin4 |
1. College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China;
2. Key Laboratory of Fish Applied Biology and Aquaculture in North China, Liaoning Province, Dalian 116023, China;
3. Liaoyang County Xingda Livestock Farm, Liaoning Province, Liaoyang 111217, China;
4. Liaoning Academy of Freshwater Fisheries, Liaoyang 111000, China |
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Abstract In order to achieve efficient reproductive regulation of fat minnow Rhynchocypris lagowskii as an excellent indigenous freshwater fish for aquaculture in northern China, the optimal injection regime, spawning inducor types and dose, injection time interval, water temperature at spawning inducing, sperm motility, male fish injection time and hatching method for artificial breeding were investigated from April 2022 to June 2024. The female broodstock weighing (56.0±5.0) g and the male broodstock weighing (37.5±4.5) g were injected and placed in plastic cages in cement tanks. The success rates were determined in the fat minnow spawners injected with the hormone by first and second times, with luteinizing hormone releasing hormone analogue-2 (LHRH-A2), domperidone (DOM) and human chorionic gonadotropin (HCG), injection intervals of 12 h, 16 h, 20 h, 24 h, 26 h, 28 h, and temperatures of 16.0 ℃, 17.0 ℃, 19.0 ℃, and 21.5 ℃. The activation rate, rapid movement time and lifespan of sperm were measured in 9 male broodstocks with body weight of (37.5±4.5) g injected with hormone at 0 h, 5 h, 8 h, 19 h, 24 h, 28 h and 29 h. The relative water consumption was determined during incubation in baskets+incubation tanks and in the incubation tank with running water. The results showed that the maximal success rate was observed under the conditions of twice injection[(90.00±2.89)%], injection at dose of LHRH-A2 20 μg/kg＋DOM 8 mg/kg+HCG 1500 IU/kg [(88.33±6.67)%], and the injection interval of 26h [(75.00±2.89)%], and water temperature of 21.5 ℃[(90.00±1.15)%]. The male fat minnow had the strongest sperm viability 24 h after hormone injection, with sperm activation rate of (97.00±1.00)%, rapid movement time of (101.90±11.56) s and a lifespan of (908.81±4.74) s. The maximal hatching efficiency was found in the pattern of "incubation basket+incubation barrel", saving water up to (40.00±5.00)%. To verify the effect of production experiments, the improved propagation method was adopted in 2024, with success rate of (86.67±1.67)%, significantly higher than those in 2022 (76.17±3.94)% and 2023 (65.00±2.89)%. The findings indicated that the efficient reproductive regulation of the artificial propagation method of fat minnow was achieved under regime of double injection with an injection interval of 26 h, at dose of LHRH-A2 20 μg/kg＋DOM 8 mg/kg+HCG 1500 IU/kg, water temperature of 21.5 ℃, male injection 2 h after the second injection of female, artificial insemination 24 h after the injection of male fish and incubation in "incubation basket+hatching barrel", which provides reference with the efficient artificial reproduction of fat minnow.
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Received: 06 December 2024
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