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| Intestinal Evacuation Characteristics and Digestive Enzyme Activity of Juvenile Rhinogobio ventralis |
| YUAN Ting, HUANG Geng, CHEN Feng, YU Fuhu, XIONG Mantang |
| Key Laboratory of Ecological Impacts of Hydraulic-Projects and Restoration of Aquatic Ecosystem, Ministry of Water Resources, Institute of Hydroecology, Ministry of Water Resources and Chinese Academy of Sciences, Wuhan 430079, China |
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Abstract To investigate the intestinal evacuation characteristics of juvenile Rhinogobio ventralis and to establish an optimal mathematical model for determining the most suitable feeding frequency, the proportions of intestinal wet and dry contents were observed in the juvenile R. ventralis with body weight (11.74±0.45) g satiately fed and anaesthetize by MS-222 at 0, 1, 2, 3, 4, 6, 8, 10 and 12 h after feeding at 19—20 ℃. The intestinal emptying time was estimated in R. ventralis at various emptying degrees of 0% (immediately satiated feeding), 25%, 50%, 75% and 99% when the activities of amylase, chymotrypsin and lipase were measured to investigate the changes in content quality and intestinal digestive enzyme activity in the process of intestinal emptying, and the curve fitting effect of linear model, exponential model and square root model on the emptying of intestinal contents. It was found that the wet and dry weight of the intestinal contents of the juvenile was significantly decreased over time, with the initial slow reduction, followed by a rapid decrease, and then leveling off. The square root model was shown to be the most suitable model for simulating the wet and dry weight evacuation processes of the intestinal contents in juvenile R. ventralis, with the wet weight intestinal evacuation rate of 0.802 g/h, and 80% intestinal evacuation time at 11.99 hours, and with the dry weight intestinal evacuation rate of 0.801 g/h, and the 80% intestinal evacuation time at 11.38 hours. During the intestinal evacuation process, the activities of amylase, chymotrypsin, and lipase in the intestine were first increased and then decreased. Based on these findings, it is recommended that the optimal feeding interval for juvenile R. ventralis be 12 hours, with feeding frequency of twice a day.
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Received: 27 August 2024
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