虹鳟幼鱼盐度驯化过程的生理响应特性

doi:10.16378/j.cnki.1003-1111.24150

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Abstract To understand the physiological response characteristics of rainbow trout Oncorhynchus mykiss juveniles during the salinity acclimatization, triploid rainbow trout juveniles weighing (40.0±6.5) g were placed in a continuous aerated circular tank with a diameter of 1.0 m and a depth of 1.2 m at initial salinity of 0, and then increased daily in 6 increments 2 days later until a salinity of 30 on the 5th day. The temporal changes in the enzyme activities related to antioxidant function, osmotic pressure regulation, and digestion were analyzed in the juvenile rainbow trout during the salinity gradient process, including superoxide dismutase (SOD) and catalase (CAT) in the liver and serum, the content of malondialdehyde (MDA), the activities of sodium-potassium ATPase (NKA) in the gill filaments and kidneys, and amylase (AMS), trypsin (TPS), and lipase (LPS) in the liver and intestines. The results showed that the content of MDA in serum and the activity of SOD were found to be significantly increased in the mid-period of salinity acclimation (P<0.05), and then to be returned to the initial level in the post-period of salinity acclimation, with the inverse relationship between activities of CAT and SOD during the salinity acclimation process. The content of MDA was shown to be significantly increased in the liver in both mid-phase and post-phase of the salinity acclimation (P<0.05), with the overall low content (less than 1.0 nmol/mg) and without significantly change in the activities of SOD and CAT in the liver, indicating that rainbow trout juveniles can eliminate excessive reactive oxygen species produced by the body′s stress during the salinity acclimation process through the synergistic action of antioxidant factors such as SOD and CAT, and that the blood is the main tissue for rainbow trout to eliminate oxidative stress and free radicals. The activities of NKA in kidneys and gills were fluctuated slightly during the salinization process, with slightly lower than the initial level at the end of the process, indicating that the later stage of salinization consumed less energy for osmotic pressure regulation and were able to adapt to higher salinity environments in rainbow trout. It was found that the activity of AMS in liver and intestine was fluctuated slightly during the salinization process and returned to the initial level and the activity of TPS increased significantly in the middle of the salinization process and returned to a level close to the initial level at the end of the process. The activity of LPS was found to be decreased significantly during the entire salinization process, significantly lower than the initial activity level at the end of the process, indicating that the salinization process led to little impact on the digestion of starch in rainbow trout juveniles, but enhanced the digestion of protein in the middle of the salinization process, suggesting that the salinization process may led to increase the demand for protein-based nutrients. The digestion of lipid-based nutrients was inhibited in rainbow trout by the salinization process. Therefore, it may be more beneficial for rainbow trout to undergo salinity acclimation by appropriately increasing the protein content of the feed and adding probiotic agents to the diet that have good lipid-degrading effects to promote the absorption and utilization of nutrients, as well as by adding appropriate amounts of antioxidants to diet to reduce the energy consumption of removing excess oxygen-free radicals from the body.

Key words： Oncorhynchus mykiss acclimatization of salinity antioxidant digestive osmotic pressure

Received: 02 September 2024

PACS:

S965.122

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	Articles by authors
	XIAO Yao
	GAO Shan
	ZHANG Guohan
	SONG Gang
	ZHANG Gaohua
	ZHAO Daqian
	JIANG Jingwei
	DONG Ying
	ZHOU Zunchun

Cite this article:

XIAO Yao,GAO Shan,ZHANG Guohan, et al. Physiological Responses of Juvenile Rainbow Trout Oncorhynchus mykiss to Salinity Acclimation[J]. Fisheries Science, 2025, 44(6): 989-996.

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https://www.shchkx.com/EN/10.16378/j.cnki.1003-1111.24150 OR https://www.shchkx.com/EN/Y2025/V44/I6/989

[1] 贾素雯,刘丽.不同规格虹鳟幼鱼对盐度梯度变化适应能力[J].河北渔业,2016(3):11-41.
[2] 刘骋跃,周演根,董亢,等.海水驯化对虹鳟鳃、肌肉和肝脏磷脂脂肪酸组成的影响[J].中国海洋大学学报(自然科学版),2018,48(11):33-41.
[3] SHEPHERD B S, DRENNON K, JOHNSON J, et al. Salinity acclimation affects the somatotropic axis in rainbow trout[J]. American Journal of Physiology. Regulatory, Integrative and Comparative Physiology,2005,288(5):1385-1395.
[4] 张伟,郭春山.三倍体虹鳟鱼的海水驯化试验[J].河北渔业,2014(4):27.
[5] 刘龙龙,罗鸣,陈傅晓,等.盐度对珍珠龙胆石斑鱼幼鱼生长及鳃肾组织学结构的影响[J].大连海洋大学学报,2019,34(4):505-510.
[6] 成智丽,孙鹏飞,罗珺,等.盐度驯化下许氏平鲉血清生化指标及渗透压的变化[J].水产科学,2021,40(3):310-317.
[7] 郭印,戴习林.急性盐度胁迫对美洲鲥幼鱼渗透调节的影响[J].水产科学,2022,41(4):676-681.
[8] CHEN C Z, LI P, WANG W B, et al. Response of growth performance, serum biochemical parameters, antioxidant capacity, and digestive enzyme activity to different feeding strategies in common carp (Cyprinus carpio) under high-temperature stress[J]. Aquaculture,2022,548:737636.
[9] ABDEL-LATIF H M R, ABDEL-TAWWAB M, KHALIL R H, et al. Black soldier fly (Hermetia illucens) larvae meal in diets of European seabass: effects on antioxidative capacity, non-specific immunity, transcriptomic responses, and resistance to the challenge with Vibrio alginolyticus[J]. Fish & Shellfish Immunology,2021,111:111-118.
[10] 纪凯,黄天晴,谷伟,等.温度和盐度对虹鳟(Oncorhynchus mykiss)“水科1号” 生长、消化酶活性和相关基因表达的影响[J].海洋与湖沼,2023,54(5):1476-1487.
[11] 韩步鹰,孟玉琼,刘小红,等.饲料蛋白质水平对三倍体虹鳟生长和肠道组织形态的影响[J].水产学杂志,2020,33(1):1-7.
[12] 付占斐,王鑫,王芳,等.不同盐度驯化方式对虹鳟和硬头鳟幼鱼存活和生长的影响[J].中国海洋大学学报(自然科学版),2020,50(7):37-43.
[13] 杨静雯,周演根,黄铭,等.盐度对虹鳟和硬头鳟幼鱼消化酶和抗氧化酶活性的比较研究[J].中国海洋大学学报(自然科学版),2019,49(3):119-128.
[14] 杨静雯,杨小刚,黄铭,等.盐度变化对虹鳟和硬头鳟抗氧化酶活性的影响[J].中国海洋大学学报(自然科学版),2021,51(6):26-33.
[15] 熊莹槐,杨静雯,董双林,等.虹鳟和硬头鳟早期幼鱼渗透生理及能量平衡的比较研究[J].中国海洋大学学报(自然科学版),2019,49(3):47-56.
[16] 巩建华,郭春阳,田喆,等.在淡水和海水环境下虹鳟血液理化指标、肌肉和肝脏的抗氧化酶活性的比较[J].生物学杂志,2016,33(4):34-37.
[17] 张晨捷,张艳亮,高权新,等.低盐胁迫对黄姑鱼幼鱼肝脏抗氧化功能的影响[J].南方水产科学,2015,11(4):59-64.
[18] 张晨捷,高权新,施兆鸿,等.低盐度和不同硫酸铜浓度对银鲳鳃离子调节酶和肝抗氧化功能的影响[J].中国水产科学,2014,21(4):711-719.
[19] 尹飞,孙鹏,彭士明,等.低盐度胁迫对银鲳幼鱼肝脏抗氧化酶、鳃和肾脏ATP酶活力的影响[J].应用生态学报,2011,22(4):1059-1066.
[20] ZENG L,AI C X, WANG Y H, et al. Abrupt salinity stress induces oxidative stress via the Nrf2-Keap1 signaling pathway in large yellow croaker Pseudosciaena crocea[J]. Fish Physiology and Biochemistry,2017,43(4):955-964.
[21] 张惠,曾霖,熊逸飞,等.盐度驯化改善大黄鱼盐度胁迫耐受性的作用机制[J].中国水产科学,2023,30(3):334-343.
[22] 李海涛.盐碱驯化对大鳞鲃(Luciobarbus capito)幼鱼生理生化及肠道微生物影响[D].上海:上海海洋大学,2022.
[23] ALIKO V, QIRJO M, SULA E, et al. Antioxidant defense system, immune response and erythron profile modulation in gold fish, Carassius auratus, after acute manganese treatment[J]. Fish & Shellfish Immunology,2018,76:101-109.
[24] SHIN H S, YOO J H, MIN T S, et al. The effects of quercetin on physiological characteristics and oxidative stress resistance in olive flounder, Paralichthys olivaceus[J]. Asian-Australasian Journal of Animal Sciences,2010,23(5):588-597.
[25] 张春燕,文登鑫,姚文祥,等.不同来源虾青素对虹鳟生长性能、肉色和抗氧化能力的影响[J].动物营养学报,2021,33(2):1008-1019.
[26] 吕小燕,李建东,张磊,等.低鱼粉饲料添加虾青素对虹鳟幼鱼生长及抗氧化能力的影响[J].饲料研究,2024,47(6):65-69.
[27] EVANS D H, PIERMARINI P M, CHOE K P. The multifunctional fish gill:dominant site of gas exchange, osmoregulation, acid-base regulation, and excretion of nitrogenous waste[J]. Physiological Reviews,2005,85(1):97-177.
[28] IMSLAND A K, FOSS A, GUNNARSSON S, et al. The interaction of temperature and salinity on growth and food conversion in juvenile turbot (Scophthalmus maximus)[J]. Aquaculture,2001,198(3/4):353-367.
[29] 庄青青.盐度胁迫下尼罗罗非鱼鳃离子细胞和Na⁺-K⁺-ATPase a1的渗透调节[D].上海:上海海洋大学,2013.
[30] 康自强,邓超准,于慧娟,等.盐度对鱼类的影响[J].福建水产,2013,35(5):395-401.
[31] ABDEL-TAWWAB M, MONIER M N. Stimulatory effect of dietary taurine on growth performance, digestive enzymes activity, antioxidant capacity, and tolerance of common carp, Cyprinus carpio L., fry to salinity stress[J]. Fish Physiology and Biochemistry,2018,44(2):639-649.
[32] 刘国庆,周萌,解绶启,等.饲料中乙醇梭菌蛋白含量对草鱼肌肉品质、抗氧化能力和相关基因表达的影响[J].中国水产科学,2023,30(2):194-205.
[33] 成永洲.海水盐度对斜带石斑鱼幼鱼生长及生理生化的影响[D].南京:南京农业大学,2015.
[34] 罗鸣钟,关瑞章,靳恒.盐度对花鳗鲡和太平洋双色鳗鲡幼鳗生长性能及消化酶活力的影响[J].水生生物学报,2015,39(4):653-660.
[35] MOUTOU K A, PANAGIOTAKI P, MAMURIS Z. Effects of salinity on digestive protease activity in the euryhaline sparid Sparus aurata L.:a preliminary study[J]. Aquaculture Research,2004,35(9):912-914.
[36] 陈品健,王重刚,郑森林.盐度影响真鲷幼鱼消化酶活力的研究[J].厦门大学学报(自然科学版),1998,37(5):754-756.