Effects of Air Exposure Stress on Survival Rate and Response to Oxidative Stress in Ridgetail White Prawn Exopalaemon carinicauda
DENG Gaowei1, DUAN Jiancheng1, WANG Yu1, WANG Linhua1, OUYANG Lefei1, GAO Huan1,2,3, LAI Xiaofang1,2,3, ZHANG Qingqi4, YAN Binlun1,2,3
1. College of Marine Sciences and Fisheries, Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang 222005, China; 2. Jiangsu Institute of Marine Resources Development, Lianyungang 222005, China; 3. The Jiangsu Provincial Platform for Conservation and Utilization of Agricultural Germplasm, Nanjing 210014, China; 4. Ganyu-Jiaxin Aquaculture Limited Company of Lianyungang, Lianyungang 222100, China
Abstract：The objective of present research was to investigate the effects of air exposure stress on survival rate and response to oxidative stress in ridgetail white prawn Exopalaemon carinicauda. The shrimps were grouped to control treatment and four experimental treatments exposed to four challenges: room temperature and air exposure, room temperature and wet, low temperature and air exposure, low temperature and wet. The accumulative survival rates were calculated within 12.0 h, and the antioxidant enzyme activities were measured within 2.5 h under air exposure stress. The results showed that the accumulative survival rates of ridgetail white prawn in the groups were significantly different. At 3.0 h, all individuals in room temperature and air exposure were dead, while the other three test groups (room temperature and wet, low temperature and air exposure, and low temperature and wet) were 6.7%, 38.9% and 40.0% respectively. Moreover, the activities of catalase (CAT) and glutathione peroxidase (GPx) in the four test groups were significantly higher than those in the control group(P<0.05). In addition, the activities of superoxide dismutase (SOD) were decreased initially and then significantly increased at 1.0 and 2.5 h (P<0.05). But, the contents of malonaldehyde (MDA) were significantly increased initially at 1.0 and 2.5 h (P<0.05) and then decreased. These findings indicated that low and wet temperature improved the survival rates of ridgetail white prawn, the response to oxidative stress of ridgetail white prawn were induced by air exposure stress. Activities of CAT, GPx, SOD and MDA were changed significantly, which can be used as indicators for monitoring the health of ridgetail white prawn exposed to air exposure conditions.
邓高威,段健诚,王玉,王林华,欧阳乐飞,高焕,赖晓芳,张庆起,阎斌伦. 干露胁迫对脊尾白虾存活率及氧化应激反应的影响[J]. 水产科学, 2021, 40(2): 244-249.
DENG Gaowei, DUAN Jiancheng, WANG Yu, WANG Linhua, OUYANG Lefei, GAO Huan, LAI Xiaofang, ZHANG Qingqi, YAN Binlun. Effects of Air Exposure Stress on Survival Rate and Response to Oxidative Stress in Ridgetail White Prawn Exopalaemon carinicauda. 水产科学, 2021, 40(2): 244-249.
[1]Xu W J, Xie J J, Shi H, et al.Hematodinium infections in cultured ridgetail white prawns, Exopalaemon carinicauda, in Eastern China[J].Aquaculture,2010,300(1):25-31. [2]高焕,赵莲,薛蓓,等.脊尾白虾线粒体基因组应答饥饿的甲基化特征分析[J].水产学报,2015,39(7):953-961. [3]王志铮,祝本强,任夙艺,等.日本囊对虾(Marsupenaeus japonicus)秋繁同生群仔虾不同耐干露性能群体间的形态表型差异[J].海洋与湖沼,2016,47(3):673-680. [4]段亚飞,董宏标,王芸,等.干露胁迫对日本囊对虾抗氧化酶活性的影响[J].南方水产科学,2015,11(4):102-108. [5]姜娜,王芳,路允良,等.干露对三疣梭子蟹抗氧化和应激能力的影响[J].水产学报,2014,38(12):1996-2004. [6]刘忠颖,刘卫东,李军,等.温度和规格对虾夷扇贝干露耐受性的影响[J].水产科学,2018,37(4):464-468. [7]李亚烜,闫丽新,于笛,等.不同规格虾夷扇贝捕后耐干露特性比较[J].水产科学,2019,38(4):443-450. [8]Valipour A, Nedaei S, Noori A, et al. Dietary Lactobacillus plantarum affected on some immune parameters, air-exposure stress response, intestinal microbiota, digestive enzyme activity and performance of narrow clawed crayfish (Astacus leptodactylus, Eschscholtz)[J].Aquaculture,2019,504:121-130. [9]王玉清,孙同秋,王冲,等.干露及温度变化对毛蚶苗种成活率的影响[J].河北渔业,2016(2):1-3. [10]夏玉秀,申旭红,任爽宁,等.不同干露条件单环刺螠的耐受性及对体表黏液特性的影响[J].大连海洋大学学报,2019,34(4):538-544. [11]刘超,郭景兰,彭张明,等.施氏獭蛤稚贝对高温和干露的耐受性研究[J].水产科学,2015,34(3):169-173. [12]姜令绪,刘群,王仁杰,等.三疣梭子蟹(Portunus trituberculatus)幼体不同干露温度下死亡率的研究[J].海洋与湖沼,2012,43(1):127-131. [13]孙煜阳,童巧琼,王文,等.厚壳贻贝在不同温度下的干露耐受性研究[J].生物学杂志,2017,34(4):42-46. [14]王琦,李健,李吉涛,等.干露胁迫对脊尾白虾(Exopalaemon carinicauda) HSP70和ferritin基因表达的影响[J].海洋与湖沼,2013,44(2):409-414. [15]齐静涛.日本对虾活体干法运输技术研究[J].齐鲁渔业,1996,13(5):47-48. [16]张义浩,梅文骧,王春琳,等.活虾蛄离水干露试验[J].浙江水产学院学报,1996,15(1):43-48. [17]平洪领,李玉全.干露对天津厚蟹水分代谢和死亡率的影响[J].湖北农业科学,2014,53(18):4377-4380. [18]Duan Y F, Liu P, Li J T, et al. Expression profiles of selenium dependent glutathione peroxidase and glutathione S-transferase from Exopalaemon carinicauda in response to Vibrio anguillarum and WSSV challenge[J].Fish & Shellfish Immunology,2013,35(3):661-670. [19]时少坤,王瑞旋,王江勇,等.盐度胁迫对近江牡蛎几种免疫因子的影响[J].南方水产科学,2013,9(3):26-30. [20]陈丽梅,刘利华,胡宏辉,等.温度突变对毛蚶不同组织抗氧化酶活性的影响[J].水产科学,2019,38(4):435-442. [21]彭士明,施兆鸿,高权新,等. 增加饲料中VC质量分数对银鲳血清溶菌酶活性及抗氧化能力的影响[J].南方水产科学,2013,9(4):16-21. [22]Papadimitriou E, Loumbourdis N S. Exposure of the frog Rana ridibunda to copper:impact on two biomarkers, lipid peroxidation, and glutathione[J].Bulletin of Environmental Contamination and Toxicology,2002,69(6):885-891. [23]刘祥,王敏,陈求稳,等.典型重金属胁迫对日本沼虾的氧化损伤及交互作用[J].生态毒理学报,2017,12(6):116-126. [24]管越强,李利,王慧春,等.低氧胁迫对日本沼虾呼吸代谢和抗氧化能力的影响[J].河北大学学报(自然科学版),2010,30(3):301-306. [25]Duan Y F, Zhang J S, Dong H B, et al. Effect of desiccation and resubmersion on the oxidative stress response of the kuruma shrimp Marsupenaeus japonicus[J].Fish & Shellfish Immunology,2016,49:91-99. [26]de Almeida E A, Bainy A C D, Dafre A L, et al. Oxidative stress in digestive gland and gill of the brown mussel (Perna perna) exposed to air and re-submersed[J].Journal of Experimental Marine Biology and Ecology,2005,318(1):21-30. [27]王海锋,成永旭,李京昊,等.干露和再入水对克氏原螯虾抗氧化应激能力的影响[J].南方水产科学,2019,15(5):69-76. [28]亢玉静,郎明远,赵文.水生生物体内抗氧化酶及其影响因素研究进展[J].微生物学杂志,2013,33(3):75-80.
2021, Vol. 40 
