Multilocus Sequence Typing and Resistance Range Determination of Yersinia ruckeri from Sturgeon Hybrid
ZHANG Xiaoli1, MA Qiangsheng1, JIAO Shizhang2, SONG Xiaoyu3, MA Xiaojun1
1. College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China; 2. Agriculture and Animal Husbandry Bureau of Yongjing County, Gansu Province, Linxia 731600, China; 3. Agricultural and Rural Bureau of Ruyang County, Henan Province, Luoyang 471200, China
Abstract：A large number of hybrid sturgeon (Acipenser schrenckii ♀ ×A. baeri ♂) with body weight of (160±20) g died at a sturgeon farm in Liujiaxia, Gansu Province, from July to August 2019, the dead fish being redness, swelling and bleeding in the cloacal pores, and occasional bleeding from the genital pores, and occasionally bleeding from the snout. The 16S rRNA gene of 60 dominant bacterial strains was sequenced from 60 diseased fish livers. The results showed that the 60 isolates were all Yersinia ruckeris. Biotype detection and multilocus sequence typing showed that 60 strains of Y. ruckeris were all biotype 2, with six known ST types ST1, ST2, ST3, ST5, ST8 and ST13. On this basis, phylogenetic analysis of six housekeeping genes of Y. ruckeris was carried out in series, the isolates from the same source showing the differences. Ten antibiotic drugs were used to determine the general resistance of 60 strains of Y. ruckeris, and a total of 9 drug resistance were observed. The findings provided data to support the drug resistance status of Y. ruckeris in hybrid sturgeon farmed in Liujiaxia, and theoretical basis for the prevention, treatment and vaccine development of Y. ruckeris disease in sturgeon.
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2021, Vol. 40 
