Screening and Identification of a Marine Actinomycete with Antibacterial and Ammonia-Nitrogen Degradation Functions
TANG Manli1, LI Shuxin2, PENG Yun1, CHEN Yingying1, WANG Yanan1, MA Guizhen1, BAO Zenghai1
1. College of Marine Life and Fisheries, Jiangsu Ocean University, Lianyungang 222005, China; 2. Department of Marine Biology, College of Oceanography, Hohai University, Nanjing 210098, China
Abstract：Using Vibrio parahemolyticus and V. vulculus as indicator bacteria, the bacteriostatic activity of 37 strains of marine actinomyces in sterile fermentation broth was determined by Oxford cup method, and the bacteriostatic activity of the strains was screened out and their bacteriostatic spectra were determined. The strains with strong bacteriostatic activity were inoculated into the culture medium with a mass concentration of ammonia nitrogen of 50 mg/L and fermented for 7 d. The concentration of ammonia nitrogen in the fermentation broth of different strains was determined by indophenol blue spectrophotometry, and the excellent strains of actinomycetes with functions of bacteriostatic and degrading ammonia nitrogen were screened out. The selected strains were identified through morphological observation, physiological and biochemical test and 16S rDNA sequence analysis. The results showed that 15 strains of actinomycetes had inhibitory effects on both V. parahaemolyticus and V. vulnificus, in which strain F28 had the strongest inhibitory effect on V. parahaemolyticus and V. vulnificus, with diameter of the inhibitory ring of (31.57±0.63) mm and (18.93±0.92) mm, respectively. In addition, the strain had a strong inhibitory effect on all 10 pathogenic bacteria tested, with a wide inhibitory spectrum. Strain F28 had strong ammonia nitrogen degradation ability with degradation rate of 46.86%. Combined with the results of bacteriostatic activity, it was concluded that strain F28 as Streptomyces mediolani had both bacteriostatic and ammonia-nitrogen degradation functions, and potential development and application prospects.
汤曼利, 李舒馨, 彭云, 陈莹莹, 王亚楠, 马桂珍, 暴增海. 1株具有抑菌和氨氮降解功能的海洋放线菌筛选与鉴定[J]. 水产科学, 2021, 40(4): 610-617.
TANG Manli, LI Shuxin, PENG Yun, CHEN Yingying, WANG Yanan, MA Guizhen, BAO Zenghai. Screening and Identification of a Marine Actinomycete with Antibacterial and Ammonia-Nitrogen Degradation Functions. 水产科学, 2021, 40(4): 610-617.
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2021, Vol. 40 
