Effects of Feed Feeding, Starvation and Live Food Refeeding on Glucose Metabolism in Mandarinfish Siniperca chuatsi
MA Chenxi, ZHAO Jinliang, ZENG Mengdong, SONG Yindu, ZHOU Yunhong
Laboratory of Freshwater Fisheries Germplasm Resource, Ministry of Agriculture and Rural Affairs, Shanghai Collaborative Innovation for Aquatic Animal Genetics and Breeding, National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China
Abstract：In order to understand the effects of nutrient levels on glucose metabolism of mandarinfish Siniperca chuatsi, a three-week feed feeding experiment, and a starvation and refeeding experiment were carried out in juvenile with average body weights of (3.27±0.63) g and (3.54±0.52) g, and growth index, liver index, liver glycogen, muscle glycogen and blood glucose contents, insulin and glucagon contents, and 9 enzyme activities in glycolysis, pentose phosphate, gluconeogenesis pathways were measured and compared. The results showed that in feed feeding group there was significantly lower growth than that in the live food group, without significant difference in blood glucose, liver glycogen, muscle glycogen, insulin and glucagon contents between two groups, most enzymatic activities did not change significantly except for phosphofructokinase (PFK), and pyruvate kinase (PK). Compared with the normal feeding group, after three weeks of starvation, body weight, liver weight and liver index were significantly decreased. Liver glycogen and muscle glycogen, and blood glucose contents were significantly declined; insulin decreased and glucagon content increased; glucose dehydrogenase (GDH) activity decreased significantly, followed by PFK and PK activities, whereas phosphoenolpyruvate carboxykinase (PEPCK) and fructose-1,6-bisphosphatase (FBPase) activities did not changed. After two weeks of starvation and one week of refeeding, liver weight and liver index recovered faster than body weight. Blood glucose recovered faster than liver glycogen and muscle glycogen levels; insulin and glucagon levels were returned to normal level; all enzymatic activity restored. These findings indicated that feed feeding had no significant effect on glucose metabolism, while a significant change on glucose metabolism happened in starvation and refeeding of mandarinfish.
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2021, Vol. 40 
