Abstract:To investigate the effect of vitamin E level on blood immune index and digestive enzyme activity of red-eared turtle Trachemys scripta elegans under high temperature stress, forty healthy juvenile red-eared turtles with initial body mass of (108.42±19.56) g were randomly divided into 4 groups at (34±0.5) ℃ for 30-day feeding trial. There were two parallels in each group, and five turtles in each parallel. The turtles in each group were fed the diets containing vitamin E at a dose of 0(control group), 150, 250 and 350 mg/kg, respectively. The activities of serum antioxidant enzymes and intestinal digestive enzymes in serum and intestinal tissues of juvenile turtles were detected respectively. The results showed that the activities of superoxide dismutase in the 250 mg/kg group and catalase in the 3 treatment groups were significantly higher than those in the control group, especially in the 250 mg/kg group. The contents of malondialdehyde in the treatment groups were significantly lower than that in the control group (P<0.01), vitamin E did not lead to affect on intestinal amylase activity significantly (P>0.05), but very significant/significant effect on protease and lipase activities (P<0.01, P<0.05).The lipase activity of the experimental groups was significantly lower than that in control group, with the minimal activity in the 250 mg/kg group. On the contrary, the activity of protease in treatment groups was significantly higher than that in the control group, with the maximal activity in 250 mg/kg group. To sum up, the addition of vitamin E in diet led to significantly improve the anti-stress ability of juevenile red-eared turtle under high temperature stress environment, and improve the protein digestibility within limits of juevenile red-eared turtle fed meat. The addition of vitamin E in 250 mg/kg was the optimal for diet of red-eared turtle.
雷金洪, 刘天天, 张广武, 傅丽容, 史海涛. 高温应激下维生素E对红耳龟免疫指标及消化酶的影响[J]. 水产科学, 2022, 41(2): 311-315.
LEI Jinhong, LIU Tiantian, ZHANG Guangwu, FU Lirong, SHI Haitao. Effect of Vitamin E on Immune Index and Digestive Enzyme Activity of Red-Eared Turtle Trachemys scripta elegans under High Temperature Stress. Fisheries Science, 2022, 41(2): 311-315.
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