Effects of Green Grass Food and Compound Feed on Growth, Digestion and Fish Quality of Hybrid between Bluntnose Black Bream Megalobrama amblycephala and Topmouth Culter Culter alburnus
LIN Xin, ZHENG Guodong, SU Xiaolei, CHEN Jie, ZOU Shuming
Genetics and Breeding Center for Blunt Snout Bream, Ministry of Agriculture and Rural Affairs, Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs, National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China
Abstract：In order to compare the growth, digestion and meat quality of hybrid bluntnose black bream Megalobrama amblycephala ♀ × topmouth culter Culter alburnus ♂ (MC)after ingesting green grass food and compound feed, MC juvenile fish were fed grass food and compound feed for 30 days, and then measured for their growth, muscle nutrients, digestive enzyme activity, digestive tract structure, and flesh taste and other indicators. The results showed that (1)there was significantly lower growth index in fish fed ryegrass Lolium perenne than that in the fish fed the compound feed, but the intestinal body index and length of the intestine were significantly higher than those in the compound feed group (P<0.05). (2)There was higher moisture in the fish in the green feed group than that in the compound feed group, but the contents of crude fat, crude protein and crude ash were lower than those in the compound feed group (P<0.05). And the muscle fiber area in the green feed group was also smaller than that in the compound feed group.(3)The intestinal trypsin and lipase activities were lower in the green feed group than those in the compound feed group, but the amylase activity was higher. The hybrid in compound feed group had more liver fat accumulation and intestinal fold goblet cells than the fish in green feed group did.(4)Electronic tongue analysis showed that the descending order of the two-dimensional intercept size of the principal component analysis (PCA) was green feed group>compound feed group.(5)Nucleotide analysis showed that the taste nucleotide of fish meat was greatly affected by feeding habits. There was higher content of 5′-AMP (sweet) and 5′-IMP (fresh) in the green feed group than that in the compound feed group, while the content of Hx (bitter) relatively small. In summary, green feed and compound feed had a greater impact on the growth and digestion of MC and the taste of fish. Fish in high-protein compound feed grew faster, while those in green feed group had better taste. The digestive enzyme activity in the intestine was changed according to the different foods ingested.
林欣, 郑国栋, 苏晓磊, 陈杰, 邹曙明. 黑麦草与配合饲料对鲂鲌杂交种生长、消化及鱼肉品质的影响[J]. 水产科学, 2022, 41(5): 769-777.
LIN Xin, ZHENG Guodong, SU Xiaolei, CHEN Jie, ZOU Shuming. Effects of Green Grass Food and Compound Feed on Growth, Digestion and Fish Quality of Hybrid between Bluntnose Black Bream Megalobrama amblycephala and Topmouth Culter Culter alburnus . Fisheries Science, 2022, 41(5): 769-777.
[1]张卓慧,陈婕,黎玲,等.动物远缘杂交研究进展[J].中国科学:生命科学,2014,44(2):161-174. [2]刘少军.远缘杂交导致不同倍性鱼的形成[J].中国科学:生命科学,2010,40(2):104-114. [3]楼允东,李小勤.中国鱼类远缘杂交研究及其在水产养殖上的应用[J].中国水产科学,2006,13(1):151-158. [4]顾志敏,贾永义,叶金云,等.翘嘴红鲌(♀)×团头鲂(♂)杂种F1的形态特征及遗传分析[J].水产学报,2008,32(4):533-544. [5]金万昆,杨建新,高永平,等.(团头鲂♀×翘嘴红鲌♂)杂种F1的含肉率、肌肉营养成分及氨基酸含量[J].淡水渔业,2006,36(1):50-51. [6]甘小顺,江海龙,张汉勤,等.杂交鲌F1代池塘生态养殖技术[J].现代农业科技,2015(18):277. [7]李武辉.鲂鲌品系和鲌鲂品系遗传特性及食性研究[D].长沙:湖南师范大学,2018. [8]冉井洪,李维玉,姚茂华.利用芜萍培育草鱼种试验[J].科学养鱼,2007(9):67-68. [9]蒋元方,袁亚华.以颗粒饲料为主的养鱼方式之弊端[J].渔业致富指南,2009(18):25-26. [10]邢磊,王仁华.浅谈青饲料在养鱼中的应用[J].江西饲料,2015(6):31. [11]翁丽萍,戴志远,赵芸,等.养殖大黄鱼和野生大黄鱼特征滋味物质的分析与比较[J].中国食品学报,2015,15(4):254-261. [12]肖雄,林淑琴,吴雄飞,等.三种不同养殖模式下大黄鱼鱼皮、鱼鳞挥发性风味成分分析[J].中国水产科学,2017,24(2):341-354. [13]CLARET A, GUERRERO L, GARTZIA I, et al. Does information affect consumer liking of farmed and wild fish?[J].Aquaculture,2016,454:157-162. [14]薛莹,金显仕.鱼类食性和食物网研究评述[J].海洋水产研究,2003,24(2):76-87. [15]GALLAGHER M L, LUCZKOVICH J J, STELLWAG E J. Characterization of the ultrastructure of the gastrointestinal tract mucosa, stomach contents and liver enzyme activity of the pinfish during development[J].Journal of Fish Biology,2001,58(6):1704-1713. [16]DREWE K E, HORN M H, DICKSON K A, et al. Insectivore to frugivore:ontogenetic changes in gut morphology and digestive enzyme activity in the characid fish Brycon guatemalensis from Costa Rican rain forest streams[J].Journal of Fish Biology,2004,64(4):890-902. [17]KRAMER D L, BRYANT M J. Intestine length in the fishes of a tropical stream:2.relationships to diet—the long and short of a convoluted issue[J].Environmental Biology of Fishes,1995,42(2):129-141. [18]ABD EL HAFEZ E A, MOKHTAR D M, ABOU-ELHAMD A S, et al. Comparative histomorphological studies on oesophagus of catfish and grass carp[J].Journal of Histology,2013,2013:858674. [19]王川.延迟首次投喂对胭脂鱼仔鱼生长的影响及其机理研究[D].重庆:西南大学,2014. [20]周兴华,向枭,向桢,等.饥饿与再投喂对中华倒刺鲃幼鱼生长和消化酶活性的影响[J].淡水渔业,2012,42(3):50-54. [21]吴婷婷,朱晓鸣.鳜鱼、青鱼、草鱼、鲤、鲫、鲢消化酶活性的研究[J].中国水产科学,1994,1(2):10-17. [22]倪寿文,桂远明,刘焕亮.草鱼、鲤、鲢、鳙和尼罗非鲫脂肪酶活性的比较研究[J].大连水产学院学报,1990,5(增刊):19-24. [23]王宏田,张培军.牙鲆体内消化酶活性的研究[J].海洋与湖沼,2002,33(5):472-476. [24]徐云峰,施晓丽,罗卫星,等.黔北麻羊主产区常用青绿饲料营养成分分析[J].贵州农业科学,2019,47(7):66-69. [25]邱伟强,谢晶,陈舜胜,等.虾类冷藏期间ATP关联产物含量的变化及其降解途径的研究[J].现代食品科技,2015,31(10):103-108. [26]蒋文枰,贾永义,刘士力,等.鲌鲂F1、F2及其亲本肌肉营养成分的比较分析[J].浙江农业学报,2020,32(7):1166-1175. [27]朱庆红,李莉.配合饲料搭配浮萍培育草鱼种高产试验[J].安徽农业科学,2009,37(16):7493-7494. [28]段小明,陈世琪,彭双智.青饲料和颗粒饲料养殖草鱼效益对比试验[J].内陆水产,2008,33(1):21-22. [29]张耀武,陈万光.不同饲料对主养草鱼池塘的效果试验[J].科学养鱼,2007(2):65-66. [30]郭建林,马恒甲,孙丽慧,等.不同精、青饲料比例对草鱼生长、形体及肌肉营养成分的影响[J].浙江海洋学院学报(自然科学版),2012,31(6):503-508. [31]李宝山,冷向军,李小勤,等.投饲蚕豆对不同规格草鱼生长、肌肉成分和肠道蛋白酶活性的影响[J].上海水产大学学报,2008,17(3):310-315. [32]柳明,朱晓鸣,雷武,等.不同饲料营养对池塘养殖长吻鮠生长性能和鱼肉品质的影响[J].水生生物学报,2010,34(3):598-610. [33]尤宏争,邵蓬,高建忠,等.出口级黄板鳅(♀)×台湾泥鳅(♂)杂交种及其亲本肌肉营养成分分析比较[J].大连海洋大学学报,2017,32(2):198-204. [34]张海涛,王安利,李国立,等. 营养素对鱼类脂肪肝病变的影响[J]. 海洋通报,2004,23(1):82-89. [35]刘立鹤,黄绮雯,胡先勤,等.不同商品饲料对鮰生长、体色及肝脏组织结构的影响[J].淡水渔业,2009,39(3):57-63. [36]原居林,郭建林,刘梅,等.不同饲料类型和放养密度对乌鳢生长特性及营养品质的影响[J].大连海洋大学学报,2017,32(5):534-543. [37]刘玥宏,丁悦,徐敬东.肠道杯状细胞及其功能研究进展[J].世界华人消化杂志,2017,25(14):1279-1286. [38]潘黔生,郭广全,方之平,等.6种有胃真骨鱼消化系统比较解剖的研究[J].华中农业大学学报,1996,15(5):63-69. [39]张小谷,洪一江,汪洪.四种淡水鱼类前肠的组织学比较研究[J].南昌大学学报(理科版),1999,23(4):339-342. [40]刘飞,张轩杰,刘少军,等.湘云鲫、湘云鲤消化道的组织学研究[J].中国水产科学,2001,8(2):23-27. [41]MORRISON C M, WRIGHT JR J R. A study of the histology of the digestive tract of the Nile tilapia[J].Journal of Fish Biology,1999,54(3):597-606. [42]PARK J Y, KIM I S. Histology and mucin histochemistry of the gastrointestinal tract of the mud loach, in relation to respiration[J].Journal of Fish Biology,2001,58(3):861-872. [43]吴金英,林浩然.斜带石斑鱼消化系统胚后发育的组织学研究[J].水产学报,2003,27(1):7-12. [44]OLATUNDE A A, OGUNBIYI O A. Digestive enzymes in the alimentary tracts of three tropical catfish[J].Hydrobiologia,1977,56(1):21-24. [45]SZABÓ E, BÖCSKEI Z, NÁRAY-SZABÓ G, et al. The three-dimensional structure of Asp189Ser trypsin provides evidence for an inherent structural plasticity of the protease[J].European Journal of Biochemistry,1999,263(1):20-26. [46]HOEHNE-REITAN K, KJØRSVIK E, REITAN K I. Bile salt-dependent lipase in larval turbot, as influenced by density and lipid content of fed prey[J].Journal of Fish Biology,2001,58(3):746-754. [47]尾崎久雄.鱼类消化生理:下册[M].李爱杰,沈宗武,译.上海:上海科学技术出版社,1985:20-41. [48]倪寿文,桂远明,刘焕亮.草鱼、鲤、鲢、鳙和尼罗罗非鱼肝胰脏和肠道蛋白酶活性的初步探讨[J].动物学报,1993,39(2):160-168. [49]林婉玲,杨贤庆,侯彩玲,等.浸渍冻结对凡纳滨对虾冻藏过程中品质的影响[J].食品科学,2014,35(10):223-229. [50]NINOMIYA K. Umami:a universal taste[J].Food Reviews International,2002,18(1):23-38. [51]BREMNER H A, OLLEY J, STATHAM J A, et al. Nucleotide catabolism:influence on the storage life of tropical species of fish from the north west shelf of Australia[J].Journal of Food Science,1988,53(1):6-11. [52]施文正,方林,吴旭干,等.我国沿海主要海域雌性三疣梭子蟹呈味成分含量的比较[J].食品科学,2017,38(16):127-133.
2022, Vol. 41 
