1. Tianjin Marine Reach Engineering Technology Center, Department of Fisheries Resources, Tianjin Fisheries Research Institute, Tianjin 300457, China; 2. National Marine Data and Information Service Centre, Marine Information Technology Innovation Center, Ministry of Natural Resources, Tianjin 300171, China
Abstract:In order to study the impact of artificial reef release on sediment bacteria, sediment bacteria detection and environmental factor determination were conducted in artificial reef area built in 2014, newly built reef area in 2018 and control area by high-throughout squencing technology in September 2018. The results showed that after two months of reef- release, the bacterial community structure in the reef release area tended to be gradually consistent with that in the newly built reef release area, and the diversity index and species number in the control area were lower than that in the reef-throwing area, with significant differences (P<0.05). Proteobacteria were the most abundant strains in Bohai Bay. The abundance of Proteobacteria and Bacteroidetes in the control area was lower than that in the other two regions. The abundance of Firmicutes and cyanobacteria in the control area was significantly higher than that in the other two regions. In terms of species, the bacterial species in the control area were significantly more than those in the other two regions, and the abundance of Sulfurovum was significantly higher than that in the other two regions. However, the abundance of Woeseia and desulfurization bacteria in the other two regions was significantly higher than that in the control area. Compared with the background value and the control area, the particle size of the sediment and the sulfide content were decreased in the artificial reef, and dissolved oxygen concentration were reduced in summer. Correlation analysis of functional genes and influencing factors showed that the sulfur reduction reaction of the reef sediment bacteria affected the reef environment, and the change of the reef environment also affected the structure of the bacterial flora.
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