Effects of Dietary Montmorillonite on Growth Performance, Digestive Enzyme Activities, and Immunity of Juvenile Sea Cucumber Apostichopus japonicus
LI Junhua1, LIU Jialiang1, LI Jing1, SUN Luping1, LIU Shilin2, WANG Yuben1
1. National Alage and Sea Cucumber Project Technology Research Centre, Shandong Oriental Ocean Sci-Tech Co., Ltd., Yantai 264003, China; 2. Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
Abstract：The juvenile sea cucumber Apostichopus japonicus with initial body weight of (1.37±0.07) g were reared in a 100 L plastic steel water tank at stocking density of 45 individuals per tank and fed diets containing 0%, 0.20%, 0.40%, 0.60% and 0.80% of montmorillonite for 75 days. Then the activities of intestinal protease and amylase and the activities of superoxide oxidase, lysozyme and alkaline phosphatase in coelomic fluid of the sea cucumber were determined at water temperature of 15.8—21.0 ℃ to probe into the effects of montmorillonite on growth performance, digestive enzyme activities, immunity and water quality in the juvenile sea cucumber culture tank. The results showed that montmorillonite supplement significantly enhanced the specific growth rate (SGR) of the juveniles, with the maximal SGR in the 0.40% supplement group (P<0.05), without significant differences in the survival rate (SR) among the groups (P>0.05). The juveniles fed the diets supplemented with montmorillonite had significantly high apparent digestibility of dry matter (P<0.05). The concentrations of montmorillonite supplement ranging from 0.20% to 0.60% led to significantly increase the activities of protease and amylase (P<0.05), and the maximum values [(73.27±3.73) U/mg and (70.63±1.96) U/mg, respectively] were both found in the 0.40% group. There were different activities of superoxide dismutase (SOD), lysozyme (LSZ) and alkaline phosphatase (AKP) in body fluid of the juveniles in different groups, with the maximal effect of montmorillonite supplement on the LSZ activity. The maximum activity of LSZ was observed in the 0.60% group, 50% higher than that in the control group. Therefore, the findings showed that diet supplemented with 0.40% montmorillonite improved the digestibility, immunity and growth of the sea cucumber, which provides foundation of optimizing the cultivation technology of sea cucumber.
李君华, 刘佳亮, 李静, 孙路平, 刘石林, 王玉本. 蒙脱石对仿刺参幼参生长、主要消化酶和免疫酶活性的影响[J]. 水产科学, 2023, 42(1): 96-101.
LI Junhua, LIU Jialiang, LI Jing, SUN Luping, LIU Shilin, WANG Yuben. Effects of Dietary Montmorillonite on Growth Performance, Digestive Enzyme Activities, and Immunity of Juvenile Sea Cucumber Apostichopus japonicus. 水产科学, 2023, 42(1): 96-101.
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2023, Vol. 42 
