Health-Promoting Effects of Bacillus licheniformis and Mulberry Leaf Extracts Compound Preparations on Yesso Scallop Patinopecten yessoensis
MI Rui1, GUAN Xiaoyan1, WANG Qingzhi1, JIANG Jingwei1, LI Hualin1, JIANG Pingzhe1,2,3, XU Yongping2,3, LI Shuying2,3, ZHOU Zunchun1
1. Liaoning Ocean and Fisheries Science Research Institute, Dalian 116023, China; 2. Post Doctoral Work Station of Dalian SEM Bio-Engineering Technology Co., Ltd., Dalian 116620, China; 3. Technology Innovation Center for Phage Application of Liaoning Province, Dalian 116620, China
Abstract：This study was conducted to investigate health-promoting effects of Bacillus licheniformis and mulberry leaf extracts compound preparations on Yesso scallop Patinopecten yessoensis. Yesso scallop were divided into different groups and fed basal diets (CK group), the basal diets supplemented with B. licheniformis (B. licheniformis treatment group), basal diets supplemented with mulberry leaf extracts (mulberry leaf extracts treatment group), or both of the two (combination treatment group). During the 30-day feeding period, the activities of digestive and immune-related enzymes were determined every ten days respectively. The results suggested that the scallops fed with supplementary diets of B. licheniformis and mulberry leaf extracts in combination produced the maximum digestive and immune activities, while the minimum values occurred in the control group. Dietary B. licheniformis and mulberry leaf extracts in combination might be more capable of improving nutrient digestion, sharpening responses to phosphatase and promoting the antioxidant abilities.The results of the challenge test showed that the cumulative mortality rate was the minimal in the combination treatment group, while the maximal mortality occurred in the control group. No significant differences were observed between the B. licheniformis treatment and mulberry leaf extracts treatment groups. Furthermore, the combination treatment group showed the lowest counts of Vibrio, while the maximum values occurred in the control group, suggesting that the combination treatment group could be more efficient at eliminating Vibrio in digestive tracts compared with others. These data collectively confirmed that a synergistic system was produced when subject to dietary supplementation of B. licheniformis and mulberry leaf extracts in combination, leading to more obvious health-promoting effects.
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2023, Vol. 42 
