Abstract：To determine the dosage regimen of doxycycline hydrochloride (DH) in treatment of Aeromonas veronii infection in yellow catfish Pelteobagrus fulvidraco, the minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), anti-drug mutation concentration (MPC), drug resistance selection window (MSW) and post antibacterial effect (PAE) of DH against A. veronii were determined in vitro. According to the application protocol of DH, yellow catfish with body weight of (200±50) g was administered orally by DH at a dose of 20 mg/kg at water temperature of (20±2) ℃, and samples of blood, muscle, brain, hepatopancreas and kidney were collected at 0.5, 1, 2, 4, 8, 12, 24, 48 and 78 h after administration for determination of the DH concentration in the tissues above by HPLC. The results showed that there was minimum inhibitory concentration of DH against A. veronii of 0.5 μg/mL, the minimum bactericidal concentration of 1 μg/mL, the anti-drug mutation concentration of 4 μg/mL, the drug resistance selection window of 0.5—4.0 μg/mL, and post antibacterial effect of (0.95±0.24), (0.79±0.58), (1.92±0.71) h, respectively. After oral administration of DH at a dose of 20 mg/kg, AUC0~78 h/MIC of 75.558 was observed in serum, 686.5486 in hepatopancreas, 557.2362 in kidney, 736.9682 in muscle and 234.9658 in brain, and ρmax/MIC of 2.4908 in serum, 18.6652 in hepatopancreas, 15.3044 in kidney, 24.159 in muscle and 10.9018 in brain. Based on the effective standard of concentration-dependent drug of AUC(0~t)/MIC＞125 and ρmax/MIC＞8 , the DH regimen with a dose of 20 mg/kg once a day led to effectively treat the infection of A. veronii in yellow catfish, and the withdrawal time for DH was at least 11.2 days. The finding provides a theoretical basis for the clinical rational use of DH in yellow catfish and the prevention and treatment of A. veronii infection in yellow catfish.
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2024, Vol. 43 
