Effects of Two Antibiotics on Intestinal Histological Structure and Tight Junction Protein Gene Expression in Large Yellow Croaker Larimichthys crocea
CHENG Haoxue1, WANG Gengshen2, XU Yexiang1, WANG Ying1, LIU Huiling1, XU Wei1, SHI Hui2, XIE Jianjun2, WANG Wei2, ZHANG Dongxu2, XU Wenjun2
1. School of Fishery, Zhejiang Ocean University, Zhoushan 316022, China; 2. Zhejiang Province Key Laboratory of Mariculture and Enhancement, Zhejiang Marine Fisheries Research Institute, Zhoushan 316021, China
Abstract：In order to explore the effects of doxycycline hydrochloride and enrofloxacin on the intestinal histological structure and expression of intestinal tight junction proteins genes in large yellow croaker Larimichthys crocea, the large yellow croaker with body weight of (41.57±1.63) g was reared in a circular plastic tank with a diameter of 1 m and water depth of 1.25 m, and fed the diets containing both drugs at a dose of 0, 0.2, 1.0 and 2.0 g/kg at water temperature of 16.80—22.50 ℃ for 5—7 days. The morphological changes in intestinal histology were observed and the relative expression levels of tight junction protein genes Claudin-7, ZO-1 and Occludin were detected in the large yellow croaker fed the medicated diets 1, 5 and 15 days after the drug was stopped to be fed. The results showed that the large yellow croaker fed the medicated diets had intestinal villus atrophy and tissue vacuolization, with gradually elevated in the degree of intestinal tissue damage with the increase in dose and prolongation of the administration time. The expression levels of the tight junction protein gene were shown to be decreased. The intestinal mucosal injury was not found to be recovered to the level of the control group 15 days after drug withdrawal, with the higher at the higher drug concentration. There was still significantly lower expression level of tight junction protein gene in the high-dose group than that in the control group at 15 days after withdrawal (P<0.05). In conclusion, doxycycline hydrochloride and enrofloxacin caused intestinal tissue damage and reduced intestinal permeability in large yellow croaker in a dose-time dependent manner,and the higher intestinal and the damage still did not fully recover 15 days after administration.
[1] 农业农村部渔业渔政管理局,全国水产技术推广总站,中国水产学会.2022中国渔业统计年鉴[M].北京:中国农业出版社,2022:22-26. [2] 朱志东,吕莉,邓剑壕,等.鱼类诺卡氏菌病的研究进展[J].水产养殖,2018,39(1):48-52. [3] 金珊,王国良,赵青松,等.海水网箱养殖大黄鱼弧菌病的流行病学研究[J].水产科学,2005,24(1):17-19. [4] HUANG L X, ZUO Y F, JIANG Q L, et al. A metabolomic investigation into the temperature-dependent virulence of Pseudomonas plecoglossicida from large yellow croaker (Pseudosciaena crocea)[J]. Journal of Fish Diseases,2019,42(3):431-446. [5] 傅超英,王建平,孙琛,等.大黄鱼主要致病菌拮抗菌株的分离鉴定、抑菌谱及安全性分析[J].生物技术通报,2019,35(1):67-75. [6] 陈洪清.一株大黄鱼致病性溶藻弧菌的耐药性及耐药基因分析[J].渔业信息与战略,2020,35(1):63-67. [7] 葛明峰,金晗,柳海,等.浙江省宁波市大黄鱼病原菌分离鉴定及其耐药性分析[J].中国动物检疫,2022,39(6):57-61. [8] 孙敏秋.网箱养殖大黄鱼内脏白点病的早期干预效果分析[J].渔业研究,2019,41(3):241-246. [9] 吴国豪.肠道屏障功能[J].肠外与肠内营养,2004,11(1):44-47. [10] 卓丽欣,赵红霞,黄燕华,等.氧化鱼油对黄颡鱼幼鱼肠道健康的影响及精氨酸的干预作用[J].水产学报,2018,42(1):100-111. [11] 刘国庆,谭青松,解绶启.新型蛋白源在水产饲料中的应用研究进展[J].饲料工业,2022,43(12):10-20. [12] 陈秀梅,王桂芹,单晓枫,等.鱼类肠道屏障损伤与肠道炎症发生发展关系的研究进展[J].河南农业科学,2022,51(5):1-9. [13] 于歌,赵倩,刘云章,等.转基因斑马鱼模型应用于索拉非尼抗肝癌活性筛选的方法研究[J].中国海洋药物,2017,36(1):48-54. [14] NUNES B, ANTUNES S C, GOMES R, et al. Acute effects of tetracycline exposure in the freshwater fish Gambusia holbrooki:antioxidant effects, neurotoxicity and histological alterations[J]. Archives of Environmental Contamination and Toxicology,2015,68(2):371-381. [15] 熊铧龙,蒋左玉,张儒学,等.盐酸恩诺沙星对杂交鲟幼鱼肝脏抗氧化酶活性的影响[J].水产科学,2017,36(5):601-605. [16] 李孟研,柴力彬,张延杰,等.微藻裂殖壶菌藻粕酶解肽抑制水产鱼类肠道炎症的功效研究[J].中国食品学报,2020,20(8):56-64. [17] LIMBU S M, ZHOU L, SUN S X, et al. Chronic exposure to low environmental concentrations and legal aquaculture doses of antibiotics cause systemic adverse effects in Nile tilapia and provoke differential human health risk[J]. Environment International,2018,115:205-219. [18] 孙春育.低聚木糖对生长中期草鱼生产性能、肠道健康的作用及其机制[D].雅安:四川农业大学,2020. [19] 涂叶绿,沙小梅,庞娟娟,等.铜镉联合胁迫处理对鲫体内重金属和组织病理的影响[J].水生生物学报,2019,43(3):545-553. [20] 吴莉芳,邢秀苹,赖红娥,等.大豆抗原蛋白Glycinin对鲤稚鱼和幼鱼肠道组织的影响[J].西北农林科技大学学报(自然科学版),2014,42(10):7-14. [21] 苗淑彦,韩蓓,胡俊涛,等.饲料中添加不同浓度四环素对乌鳢生长性能、肠道菌群组成和组织形态的影响[J].动物营养学报,2019,31(12):5813-5822. [22] 郑婷婷,涂宗财,唐平平,等.水环境中磺胺嘧啶胁迫下异育银鲫的组织病理学研究[J].水生生物学报,2018,42(1):47-56. [23] 孙永旭,董宏标,段亚飞,等.鱼类肠道应激及其损伤防护研究进展[J].海洋湖沼通报,2019(3):174-183. [24] UNI Z, GAL-GARBER O, GEYRA A, et al. Changes in growth and function of chick small intestine epithelium due to early thermal conditioning[J]. Poultry Science,2001,80(4):438-445. [25] ZEISEL M B, DHAWAN P, BAUMERT T F. Tight junction proteins in gastrointestinal and liver disease[J]. Gut,2019,68(3):547-561. [26] TURNER J R. Intestinal mucosal barrier function in health and disease[J]. Nature Reviews Immunology,2009,9(11):799-809. [27] YU J, LIU F H, YIN P, et al. Involvement of oxidative stress and mitogen-activated protein kinase signaling pathways in heat stress-induced injury in the rat small intestine[J]. Stress,2013,16(1):99-113. [28] SUZUKI T. Regulation of the intestinal barrier by nutrients:the role of tight junctions[J]. Animal Science Journa,2020,91(1):e13357. [29] SÁNCHEZ DE MEDINA F, ROMERO-CALVO I, MASCARAQUE C, et al. Intestinal inflammation and mucosal barrier function[J]. Inflammatory Bowel Diseases,2014,20(12):2394-2404. [30] 杨菊,邓俊良,夏江英,等.维生素A对大豆7S球蛋白致仔猪肠上皮细胞屏障功能损伤的影响[J].浙江农业学报,2021,33(11):2026-2033. [31] FURUSE M, HATA M, FURUSE K, et al. Claudin-based tight junctions are crucial for the mammalian epidermal barrier:a lesson from claudin-1-deficient mice[J]. The Journal of Cell Biology,2002,156(6):1099-1111. [32] WU W C, WANG S S, LIU Q, et al. Metformin protects against LPS-induced intestinal barrier dysfunction by activating AMPK pathway[J]. Molecular Pharmaceutics,2018,15(8):3272-3284. [33] 孔瑶瑶,马秀华,麦康森,等.饲料营养素对鱼类肠道紧密连接蛋白闭锁小带蛋白-1影响的研究进展[J].动物营养学报,2020,32(11):5081-5088. [34] MCCLANE B A. The complex interactions between Clostridium perfringens enterotoxin and epithelial tight junctions[J]. Toxicon,2001,39(11):1781-1791. [35] ZHANG Q Y, CHENG L, WANG J J, et al. Antibiotic-induced gut microbiota dysbiosis damages the intestinal barrier,increasing food allergy in adult mice[J]. Nutrients,2021,13(10):3315. [36] 姚鹏,郝娜.肠屏障功能与消化系统疾病关系的研究进展[J].中国中西医结合外科杂志,2021,27(5):785-787.
2024, Vol. 43 
