1. Liaoning Ocean and Fisheries Science Research Institute, Dalian 116023, China; 2. Key Laboratory of Protection and Utilization of Aquatic Germplasm Resource, Ministry of Agriculture and Rural Affairs, Dalian 116023, China; 3. Key Laboratory of Germplasm Improvement and Fine Seed Breeding for Marine Aquatic Animals, Liaoning Province, Dalian 116023, China; 4. Ocean University of China, Qingdao 266100, China
Abstract：In order to evaluate the application of the "purebred first and hybridized later" germplasm improvement technique in yesso scallop Patinopecten yessoensis, a combining ability analysis of survival traits during the larval and adult stages was conducted by employing a 3×3 complete diallel cross design consisting of the nine possible combinations of parents and their reciprocal crosses. The offsprings of three selfing F1 families (21#, 12#, and 16#) were cultured and the survival rates were evaluated in each combination. The results showed that there were significant differences (P＜0.05) in survival rates among different combinations at all stages except for the fertilization rate during the larval stage. The variations in the size and direction of the general combining abilities (GCA) were observed in the three parents, with significant differences at days 27, 140, 200, and 440, especially at day 140 with effective value of -6.88—8.25. Furthermore, the specific combining abilities (SCA) and observed 62.96% opposite directions were investigated between different mating combinations and their reciprocal crosses during the larval stage, with the maximal SCA value of 18.33 in 16#×12#. During the adult stage, the size and direction of SCA were also varied with decreasing effect values from the larval to adult stages (27—140 d). Overall, the effectiveness of the "purebred first and hybridized later" breeding strategy was shown a hybrid advantage in survival traits, particularly in the 21#×12# and 16#×12# combinations. Additionally, a strong efficiency in purge genetic load was found in yesso scallop with 21#×21#, 12#×12#, and 16#×16#, providing theoretical basis and data support for genetic improvement of yesso scallop.
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2024, Vol. 43 
