Effects of Acute Temperature Stress on Antioxidant Enzyme Activities and Non-Specific Immune Function in Juvenile Bullhead Fish Pseudobagrus ussuriensis
CHEN Junping1, WU Huihui1, SHEN Fangfang1, ZHANG Jiaxin1, YU Ruomeng1, FU Yongjie1, ZHAO Daoquan1,2,3, XIE Guoqiang2, HE Yiqing4, LI Xiujie1
1. Changyuan Branch, Henan Academy of Agricultural Sciences, Xinxiang 453400, China; 2. Henan Academy of Fisheries Science, Zhengzhou 450044, China; 3. Yiluo River Aquatic Biology Field Scientific Observation and Research Station,Huanghe River Basin, Henan Province, Sanmenxia 477000, China; 4. Bureau of Agriculture and Rural Affairs of Changyuan City, Xinxiang 453400, China
Abstract：In order to explore the effects of sharp change in water temperature on antioxidant and non-specific immune functions of juvenile bullhead fish Pseudobagrus ussuriensis, bullhead juveniles with body weight of (2.3±0.3) g were rapidly exposed to 18 ℃, 26 ℃ (control group) and 33 ℃ water stress for 24 h from 26 ℃ water in a fibergrass breeding bucket with radius of 1 m and height of 1 m, and then subjected to recovery at 26 ℃ water for 48 h. The activities of superoxide dismutase (SOD), catalase(CAT), lysozyme(LZM), alanine aminotransferase (ATF), and aspartate aminotransferase(ASF), and contents of glutathione(GSH), malondialdehyde (MAD)and total protein were detected in the stress period of 0, 3, 6, 12, and 24 h and recovery period of 3, 6, 12, and 24 h in the whole fish samples. The results showed that the activities of SOD and CAT were continuously increased during the stress period in the experimental groups, significantly higher than those in the control group (P<0.05). There was significantly higher content of malondialdehyde in the experimental groups than that in the control group at 6 and 24 h (P<0.05), while GSH content was inhibited, significantly lower than in the control group (P<0.05). At the end of the recovery period, SOD and GSH activities and MAD contents were not found to be significantly different in all experimental groups from those in control group (P>0.05), with significantly higher CAT activity in 33 ℃ group than those in control group (P<0.05). During the stress period, the activities of ATF and ASF were shown to be increased first and then decreased in all experimental groups, with significantly higher ATF activity in 18 ℃ and 33 ℃ groups than those in control group (P<0.05). The acute temperature change led to significantly decrease the LZM activity in the 18 ℃ group (P<0.05), and significantly increase the LZM activity in the 33 ℃ group (P<0.05). At the end of the recovery period, the ASF and LZM activities recovered to the control level in all experimental groups. There was significantly higher ASF activity in 33 ℃ group than that in control group (P<0.05). It was found that the acute temperature stress led to significant impact on the antioxidant enzymes and non-specific immune function of the juvenile Pseudobagrus ussuriensis, in which the high temperature stress caused irreversible damage to the immune function. It is recommended to pay more attention to changes in water temperature in daily aquaculture to avoid sharp temperature changes causing damage to the juvenile fish of P. ussuriensis.
[1] 潘伟志,陈军,王鹏,等.乌苏里拟鲿人工繁育技术研究[J].水产学杂志,2006,19(1):31-35. [2] 马本贺,王海华,马爱军,等.温度对金红色大鳞副泥鳅(Paramisgurnus dabryanus)孵化、仔鱼活力和性别分化的影响[J].海洋与湖沼,2022,53(2):448-456. [3] JIANG X Y, DONG S L, LIU R X, et al. Effects of temperature,dissolved oxygen, and their interaction on the growth performance and condition of rainbow trout (Oncorhynchus mykiss)[J]. Journal of Thermal Biology,2021,98:102928. [4] DAWOOD M A O, ALKAFAFY M, SEWILAM H. The antioxidant responses of gills, intestines and livers and blood immunity of common carp (Cyprinus carpio) exposed to salinity and temperature stressors[J]. Fish Physiology and Biochemistry,2022,48(2):397-408. [5] ISLAM M J, KUNZMANN A, SLATER M J. Responses of aquaculture fish to climate change-induced extreme temperatures:a review[J]. Journal of the World Aquaculture Society,2022,53(2):314-366. [6] CHEN C Z, LI P, WANG W B, et al. Response of growth performance, serum biochemical parameters, antioxidant capacity, and digestive enzyme activity to different feeding strategies in common carp (Cyprinus carpio) under high-temperature stress[J]. Aquaculture,2022,548:737636. [7] WU Y W, YOU X P, SUN W Q, et al. Insight into acute heat stress on meat qualities of rainbow trout (Oncorhynchus mykiss) during short-time transportation[J]. Aquaculture,2021,543:737013. [8] DE M, GHAFFAR M A, NOOR N M, et al. Effects of water temperature and diet on blood parameters and stress levels in hybrid grouper (Epinephelus fuscoguttatus ♀ × E.lanceolatus ♂) juveniles[J]. Aquaculture Reports,2019,15:100219. [9] 周明华,余祥胜,强晓刚,等.温度对乌苏里拟鲿受精孵化及仔鱼生长的影响[J].水产养殖,2019,40(10):39-43. [10] 田宏杰,庄平,高露姣.生态因子对鱼类消化酶活力影响的研究进展[J].海洋渔业,2006,28(2):158-162. [11] BOWDEN T J. Modulation of the immune system of fish by their environment[J]. Fish & Shellfish Immunology,2008,25(4):373-383. [12] HALLIWELL B, GUTTERIDGE J M C. Free Radicals in Biology and Medicine [M]. Oxford: Clarendon Press, 1985:356-364. [13] LIVINGSTONE D R. Contaminant-stimulated reactive oxygen species production and oxidative damage in aquatic organisms[J]. Marine Pollution Bulletin,2001,42(8):656-666. [14] 宁军号,秦宇博,胡伦超,等.水温骤降和缓降胁迫对褐篮子鱼血液生理生化指标的影响[J].大连海洋大学学报,2017,32(3):294-301. [15] DAGOUDO M, MUTEBI E T, QIANG J, et al. Effects of acute heat stress on haemato-biochemical parameters,oxidative resistance ability, and immune responses of hybrid yellow catfish (Pelteobagrus fulvidraco × P. vachelli) juveniles[J]. Veterinary Research Communications,2023,47(3):1217-1229. [16] 谢明媚,彭士明,张晨捷,等.急性温度胁迫对银鲳幼鱼抗氧化和免疫指标的影响[J].海洋渔业,2015,37(6):541-549. [17] 范耘硕,邵蓬,贾旭颖,等.温度胁迫对大鳞副泥鳅抗氧化与非特异性免疫指标的影响[J].渔业科学进展,2019,40(2):58-64. [18] 赵道全,李先明,谢国强,等.乌苏里拟鲿的人工繁殖技术总结[J].河南水产,2019(1):5-8. [19] 吴宜东. 乌苏里拟鲿鱼种培育和网箱养殖技术探讨 [J]. 中国水产, 2016(8): 95-98. [20] 马国军,王裕玉,石野,等.乌苏里拟鲿稚鱼饲料中蛋白质的适宜水平[J].动物营养学报,2012,24(1):176-182. [21] GUDERLEY H. Metabolic responses to low temperature in fish muscle[J]. Biological Reviews,2004,79(2):409-427. [22] 孙鹏,柴学军,尹飞,等.运输胁迫下日本黄姑鱼肝脏抗氧化系统的响应[J].海洋渔业,2014,36(5):469-474. [23] SCHLEGER I C, PEREIRA D M C, RESENDE A C, et al. Cold and warm waters:energy metabolism and antioxidant defenses of the freshwater fish Astyanax lacustris (Characiformes:Characidae) under thermal stress[J]. Journal of Comparative Physiology B,2022,192(1):77-94. [24] CHEN Y Y, HUANG X H, WANG J Z, et al. Effect of pure microcystin-LR on activity and transcript level of immune-related enzymes in the white shrimp (Litopenaeus vannamei)[J]. Ecotoxicology,2017,26:702-710. [25] 袁新程,蒋飞,施永海,等.高温胁迫对美洲鲥1+龄鱼种抗氧化与非特异性免疫相关指标的影响[J].浙江大学学报(农业与生命科学版),2021,47(1):107-117. [26] 陆健,张佳佳,王佩佩,等.急性温度胁迫对大口黑鲈存活率及肝脏生化指标的影响[J].淡水渔业,2020,50(2):87-93. [27] 王伟,姜志强,孟凡平,等.急性温度胁迫对太平洋鳕仔稚鱼成活率、生理生化指标的影响[J].水产科学,2012,31(8):463-466. [28] 王娜,邵晨,颉志刚,等.低温胁迫下虎纹蛙的生存力及免疫和抗氧化能力[J].生态学报,2012,32(11):3538-3545. [29] MARTÍNEZ-ÁLVAREZ R M, MORALES A E, SANZ A. Antioxidant defenses in fish:biotic and abiotic factors[J]. Reviews in Fish Biology and Fisheries,2005,15(1):75-88. [30] 李大鹏,刘松岩,谢从新,等.水温对中华鲟血清活性氧含量及抗氧化防御系统的影响[J].水生生物学报,2008,32(3):327-332. [31] ZHANG R Q, CHEN Q X, ZHENG W Z, et al. Inhibition kinetics of green crab (Scylla serrata) alkaline phosphatase activity by dithiothreitol or 2-mercaptoethanol[J]. The International Journal of Biochemistry & Cell Biology,2000,32(8):865-872. [32] MOURENTE G, TOCHER D R, DIAZ E, et al. Relationships between antioxidants, antioxidant enzyme activities and lipid peroxidation products during early development in Dentex dentex eggs and larvae[J]. Aquaculture,1999,179(1/2/3/4):309-324. [33] 张晨光,丁炜东,曹哲明,等.急性高温胁迫对翘嘴鳜幼鱼抗氧化酶和消化酶活性及热休克蛋白基因表达的影响[J].南方农业学报,2021,52(3):815-826. [34] 孙学亮,邢克智,陈成勋,等.急性温度胁迫对半滑舌鳎血液指标的影响[J].水产科学,2010,29(7):387-392. [35] LEE S M. Review of the lipid and essential fatty acid requirements of rockfish (Sebastes schlegeli)[J]. Aquaculture Research,2001,32:8-17. [36] TEJPAL C S, PAL A K, SAHU N P, et al. Dietary supplementation of L-tryptophan mitigates crowding stress and augments the growth in Cirrhinus mrigala fingerlings[J]. Aquaculture,2009,293(3/4):272-277. [37] 强俊,杨弘,王辉,等.急性温度应激对吉富品系尼罗罗非鱼(Oreochromis niloticus)幼鱼生化指标和肝脏HSP70 mRNA表达的影响[J].海洋与湖沼,2012,43(5):943-953. [38] 施兆鸿,张艳亮,高权新,等.云纹石斑鱼幼鱼血清生化指标对低温胁迫的响应[J].生态学杂志,2015,34(8):2222-2228. [39] SAURABH S, SAHOO P K. Lysozyme:an important defence molecule of fish innate immune system[J]. Aquaculture Research,2008,39(3):223-239. [40] JUNG T S, DEL CASTILLO C S, JAVAREGOWDA P K, et al. Seasonal variation and comparative analysis of non-specific humoral immune substances in the skin mucus of olive flounder (Paralichthys olivaceus)[J]. Developmental & Comparative Immunology,2012,38(2):295-301. [41] SIEROSLAWSKA A, RYMUSZKA A, VELISEK J, et al. Effects of microcystin-containing cyanobacterial extract on hematological and biochemical parameters of common carp (Cyprinus carpio L.)[J]. Fish Physiology and Biochemistry,2012,38(4):1159-1167. [42] 管敏,张厚本,王龙,等.急性低温胁迫对史氏鲟幼鱼抗氧化和免疫指标的影响[J].淡水渔业,2018,48(6):17-22. [43] LANGSTON A L, HOARE R, STEFANSSON M, et al. The effect of temperature on non-specific defence parameters of three strains of juvenile Atlantic halibut (Hippoglossus hippoglossus L.)[J]. Fish & Shellfish Immunology,2002,12(1):61-76. [44] 黄成裕,高世科,张家锐,等.急性温度胁迫对双带隐带丽鱼免疫指标及相关酶活性的影响[J].淡水渔业,2022,52(3):59-65.
2024, Vol. 43 
