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| Isolation and Identification of Bacterial Pathogen Aeromonas hydrophila and Development of the Inactivated Vaccine in Japanese Flounder Paralichthys olivaceus Diseased with Ascides |
| SUN Yi-min,LI Nan,SONG Jie,LI Xiao-yue,SHEN Hong-qi,WANG Feng-min,LI Quan-zhen,CAO Jie-ying,ZHAO Bao-hua |
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Abstract The pathogenic bacteria were isolated from diseased Japanese flounder Paralichthys olivaceus, and identified as Aeromonas hydrophila by biochemical methods and polymerase chain reaction based on the 16s rDNA sequence on GenBank via the homologies of nucleic acids of 99.8% between the isolated strain and the standard strains. The mice and the flounder were immunized with the inactivated vaccine made by this isolated Aeromonas hydrophila via dipping immersion and showed protection rate of 90% and 80% with the incidence of 6%. The antibody level was detected by agglutination reaction and indirect enzyme-linked immunosorbent assay (ELISA) with the antiserum seemed immunogenic crucially. The anatomized results showed that the immunized mice were healthy and the unimmunized mice (as the control) were diseased in liver, indicating that the vaccine was effective.
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Received: 25 November 2009
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