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| Effects of Salinity on Immunity-related Indicators and Digestive Enzyme Activities in Asian Swimming Crab Charybdis j aponica |
| XU Xinghong,YAN Binlun,XU Jiatao,LU Jianping,ZHOU Li,LIU Yanqing,DONG Yanqiu,ZHANG Yanqiu |
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Abstract The changes in some immunity-related indicators and digestive enzyme activities were tested in hemolymph and hepatopancreas of Asian swimming crab Charybdis j aponica collected on 0 ,0 .5 ,1 ,2 ,3 , 4 and 5 days under various salinities of 15 ,20 ,25 ,30 and 35 to evaluate the effects of salinity on density of hemocyte (DHC ) , hemocyanin content , activities of the phenoloxidase (PO ) , lysozyme (LSZ ) , superoxide dismutase (SOD) and catalase (CAT ) ,and malondialdehyde (MDA ) content and activities of acid protease ,alkali protease ,lipase ,amylase and cellulase .The results showed that DHC was found to be correlated positively with salinity ,no significant effect on hemocyanin content .The PO activity at a salinity of 30 and LSZ activity at a salinity of 20 and 30 were shown to be increase first and then decline , while the PO activity in a salinity of 15 ,20 and 35 ,and the LSZ activity in a salinity of 15 and 35 were first decreased , then increased , and dropped again . After 5 days , the activities of PO and LSZ were descendantly arranged as salinity 25 > 20 > 30 > 15 > 35 . The activities of SOD and CAT were first increased and then decreased ,the opposite tendency being the change in MDA content .On day 5 ,the activities of SOD and CAT were lower in a salinity of 15 and 35 than those in other treatments with higher MDA content (P < 0 .05) .The maximal activities of amylase and cellulase were observed in a salinity of 20 ,the maximal activities of acid protease and lipase in a salinity of 25 ,and the maximal alkali protease in a salinity of 30 .The minimal activities of 5 digestive enzymes were found in a salinity of 15 .The findings indicated that Asian swimming crab had a suitable salinity of 20 ~ 30 for immunity ,with significantly lower activities of digestive enzymes under a salinity of 15 and 35 .It is suggested that variation in activity of SOD and content of MDA be used as an indicator for immune state of Asian swimming crab under different salinity .
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Received: 25 July 2013
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