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| Acute Toxicity of 3 Disinfectants to Postlarval Puffer Fish ,Takifugu fasciatus |
| SUN Zhenlong,ZHU Yongxiang,CHEN Yunyan,LIU Dayong,GUO Zhenglong |
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Abstract The acute toxicity of benzalkonium bromide ,glutaraldehyde and povidone-iodine to postlarval puffer fish (Takifugu fasciatus) with total length of (1 .73 ± 0 .32) cm and body weight of (0 .19 ± 0 .01) g was studied by 96h semi-static bioassays under conditions of water temperature of 21—23 ℃ ,pH 8 .0—8 .5 and dissolved oxygen level of 6 .00—7 .50 mg/L .The results showed that the medium lethal concentrations (LC50 ) of benzalkonium bromide to the postlarvae were 1 .04 mg/L for 24 h ,0 .95 mg/L for 48 h ,0 .72 mg/L for 72 h and 0 .53 mg/L for 96 h;LC50 of glutaraldehyde 4 .27 mg/L for 24 h ,3 .55 mg/L for 48 h ,2 .94 mg/L for 72 h and 2 .40 mg/L for 96 h and LC50 of povideone-iodine 269 .80 mg/L for 24 h ,200 .00 mg/L for 48 h ,158 .54 mg/L for 72 h and 151 .09 mg/L for 96 h .The safe concentration was found to be 0 .24 mg/L in benzalkonium bromide , 0 .74 mg/L in glutaraldehyde and 32 .97 mg/L in povidone-iodine , higher than the concentrations recommended by the disinfectant manufacturers , indicating that the 3 disinfectants were suitable for the breeding and aquaculture of the puffer fish . However ,the application of benzalkonium bromide and glutaraldehyde was paid more attention due to the fact that the puffer fish postlarvae were sensitive to the disinfectants .
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Received: 25 May 2015
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