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Abstract In present study ,MAPKK5 (Ls-MAPKK5 ,GenBank accession number KM657202) was cloned in humphead snapper (Lutjanus sanguineus) using RACE method based on the corresponding EST ,which was obtained from cDNA library constructed using head kidney of humphead snapper in our laboratory . The full-length cDNA of Ls-MAPKK5 was 2632 bp containing an open reading frame of 1317 bp encoding 438 amino acids with an estimated molecular weight of 49 .29 ku and a theoretical pI of 6 .12 . The prognosis by SignalP 4 .0 ,TMHMM Server 2 .0 and SoftBerry-Psite revealed that the deduced amino acid of Ls-MAPKK5 did not contain a signal peptide or a transmembranous region ,but had 22 phosphorylation sites .Amino acid alignment showed that Ls-MAPKK5 possessed a PB1 and S_TKc domain .Phylogenetic tree by NJ method using MEGA5 .0 indicated that Ls-MAPKK5 protein clustered closely with Stegastes partitus and Nile tilapia Oreochromis niloticus MAPKK5 protein .
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Received: 25 May 2015
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