Effects of Acute High-Temperature Stress on Survival, Enzyme Activity, and HSP90 in Bluefin Leatherjack Thamnaconus septentrionalis
ZHANG Guangming1, CHEN Siqing2, BIAN Li2, YAN Junli1, CHEN Ying2,3, SHI Ying1, DONG Yichao1, PANG Jicai1, JIA Sifeng4, XIE Hongmei1, ZHANG Xiaojing1
1. Shandong Vocational Animal Science and Veterinary College, Weifang 261000, China; 2. Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China; 3. College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China; 4. Qilu Animal Health Products Co., Ltd., Jinan 250100, China
Abstract：In order to explore the physiological response mechanism of bluefin leatherjack Thamnaconus septentrionalis to different temperatures, juveniles bluefin leatherjack were kept in plastic buckets with a diameter of 60 cm and a capacity of 136 L at water temperature of 20 ℃ (control group), 25, 28, 31 ℃ or 33 ℃ (to determine the survival rate) for determination of survival rate, activities of superoxide dismutase (SOD), catalase (CAT), alkaline phosphatase (AKP), acid phosphatase (ACP) and heat shock protein 90 (HSP90) gene expression. Results showed that the 24,48,72,96 h median lethal temperatures (LT50) for juvenile bluefin leatherjack were 31.7, 31.5, 31.2, and 31.1 ℃, respectively. T-AOC and enzyme activities were shown to be an initial increase, then decline under thermal stress compared to the fish in control group, with SOD activity peak at 28 ℃, and the maximum levels at 25 ℃ for other enzymes. HSP90 gene expression was found to be upregulated and subsequently downregulated, with the maximal expression level in the liver at 28 ℃ (21.6-fold higher than htat in the control). The short-term stress revealed that the optimal temperature was ranged from 25 to 28 ℃ for bluefin leatherjack, with avoidance in prolonged exposure above 28 ℃. It was found that HSP90 played pivotal role in mediating physiological adaptations to thermal stress, suggesting its utility as a biomarker for evaluating heat stress responses in this species. The finding provides critical insights to support sustainable aquaculture practices for bluefin leatherjack.
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2026, Vol. 45 
