Abstract：To investigate the efficient eradication of ciliate Pseudocohnilembus persalinus by catalytic ozone oxidation technology, the ciliate in exponential growth phase was diluted in sterilized seawater with salinity of 30 to a ciliate density of 10 000 ind./mL. Then, 300 mL of the diluted ciliate suspension was injected into a fixed-bed ozone catalytic reactor, with O3 at rates of 10 g/h and 20 g/h, and catalysts including zirconia, magnesia, and alumina were added to evaluate the killing effect on P. persalinus. The results showed that the complete inactivation of P. persalinus in exponential growth phase was observed in 110 s at 10 000 ind./mL under ozone alone (10 g/h output) from a fixed-bed catalytic ozonation reactor and ozone supplied by an air-source generator. When zirconia, magnesium oxide, and aluminum oxide were utilized as catalysts under identical conditions, the inactivation was found to be reduced to 80 s, 70 s, and 40 s, respectively. Notably the eradication of 100% were found in only 10 s with the aluminum oxide catalyst, when the ozone output was elevated to 20 g/h. Kinematic observations revealed a progressive deceleration and eventual cessation of ciliary movement during treatment, and ciliary detachment, membrane rupture, and cytoplasmic leakage in the parasite. Cell Counting Kit-8 assays further validated that aluminum oxide-catalyzed ozonation had superior inhibitory effects on cell proliferation and induced significantly higher DNA degradation compared to ozone alone and in control groups. The finding confirms that catalytic ozonation enables efficient, rapid, and complete killing of P. persalinus in seawater systems.
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2026, Vol. 45 
