Abstract：In order to compare the structural characteristics of mitochondrial genomes and phylogenetic relationships between Chuanchia labiosa and Schizopygopsis pylzovi, so as to provide reference basis for the molecular identification and convergent evolution of endemic fishes in the upper reaches of the Yellow River, the complete mitochondrial genomes were sequenced, assembled, annotated, and then bioinformatics analysis was carried out in C. labiosa collected from the Xinglongshan section of the Wanchuan River, a tributary of the Yellow River and S. pylzovi collected from the Yongdeng section of the Zhuanglang River, a first-level tributary of the Yellow River by Illumina sequencing technology. The results showed that C. labiosa had the complete mitochondrial genome of 16 765 bp in length, containing 22 tRNA genes, 13 protein-coding genes, 2 rRNA genes, and 1 D-Loop control region, with nucleotides A+T content of 55.51% and the G+C content of 44.49%, indicating an obvious AT bias. While S. pylzovi had the complete mitochondrial genome of 16 746 bp in length, containing 22 tRNA genes, 13 protein-coding genes, 2 rRNA genes, and 1 D-Loop control region, with nucleotides A+T content of 55.48% and the G+C content of 44.52%, also showing an obvious AT bias. The results of phylogenetic analysis indicated that the fishes in Schizothoracinae were divided into two main clades, in which Schizopygopsis pylzovi had a relatively close evolutionary relationship with Gymnocypris eckloni chilianensis, while Chuanchia labiosa clustered with Gymnocypris przewalskii, showing a relatively close genetic relationship. The findings led to the difficult problem of morphological identification of special fishes to be solved and to offer important theoretical basis and basic data for understanding of convergent evolution and biodiversity conservation of fishes in Schizothoracinae.
王廷宽, 刘哲, 权金强, 张明盩, 柴成举, 马玲玲, 宋国林, 谢步忠, 刘志俊. 骨唇黄河鱼与黄河裸裂尻鱼线粒体全基因组特征比较分析[J]. 水产科学, 2026, 45(2): 173-185.
WANG Tingkuan, LIU Zhe, QUAN Jinqiang, ZHANG Mingzhou, CHAI Chengju, MA Lingling, SONG Guolin, XIE Buzhong, LIU Zhijun. Characteristics and Phylogenetic Analysis of Mitochondrial Genome between Chuanchia labiosa and Schizopygopsis pylzovi. Fisheries Science, 2026, 45(2): 173-185.
[1] 武云飞.中国裂腹鱼亚科鱼类的系统分类研究[J]. 高原生物学集刊,1984(3):119-140. [2] FORTELIUS M, ERONEN J T, KAYA F, et al. Evolution of Neogene mammals in Eurasia:environmental forcing and biotic interactions[J]. Annual Review of Earth and Planetary Sciences,2014,42:579-604. [3] 赵海林.渭河源头特有鱼类国家级水产种质资源保护区水生生物调查报告[J].现代农业研究,2023(3):147-150. [4] HE D K, LI C H, TAO J, et al. The complete mitochondrial genome sequence of Chuanchia labiosa (Cypriniformes:Cyprinidae)[J]. Mitochondrial DNA. Part A, DNA Mapping, Sequencing, and Analysis,2016,27(3):1638-1639. [5] 夏云.不同地理种群黄河裸裂尻鱼遗传多样性研究[D].武汉:武汉轻工大学,2017. [6] 陈彦伶,杨焕超,李青芝,等.黄河裸裂尻卵黄囊期仔鱼形态发育研究[J].四川农业科技,2021(8):41-43. [7] 娄晋铭,张智,王太,等.甘肃省3种裂腹鱼遗传多样性与地理种群分化[J].华中农业大学学报,2019,38(4):77-84. [8] XIA Y, ZHENG Y C, MURPHY R W, et al. Intraspecific rearrangement of mitochondrial genome suggests the prevalence of the tandem duplication-random loss (TDLR) mechanism in Quasipaa boulengeri [J]. BMC Genomics,2016,17(1):965. [9] 程佩琳,俞丹,刘焕章,等.基于线粒体基因组全序列的鲟形目鱼类(Pisces:Acipenseriformes)的分子系统发育重建[J].水生生物学报,2021,45(3):487-495. [10] PÉREZ-RODRÍGUEZ R, DOMÍNGUEZ-DOMÍNGUEZ O, PÉREZ PONCE DE LEÓN G, et al. Phylogenetic relationships and biogeography of the genus Algansea Girard (Cypriniformes:Cyprinidae) of central Mexico inferred from molecular data[J]. BMC Evolutionary Biology,2009,9:223. [11] 孔艳,张哲,李岩,等.后生动物线粒体基因组基因次序重组规律分析[J].干旱区研究,2014,31(2):336-341. [12] BOORE J L. Animal mitochondrial genomes[J]. Nucleic Acids Research,1999,27(8):1767-1780. [13] PENG Z G, WANG J, HE S P. The complete mitochondrial genome of the helmet catfish Cranoglanis bouderius (Siluriformes:Cranoglanididae) and the phylogeny of otophysan fishes[J]. Gene,2006,376(2):290-297. [14] 曾圣,陈小红,钟珅,等.长汀吻虾虎鱼线粒体基因组及系统发育研究[J].渔业研究,2025,47(4):395-407. [15] 马天竺,王云鹏,王起,等.脂鲤科3个种的线粒体全基因组特征及其系统发育关系[J].浙江海洋大学学报(自然科学版),2025,44(1):10-19. [16] 宋丹,彭頔,程起群.裂腹鱼亚科系统发育与适应性进化及祁连山裸鲤的分类地位[J].中国水产科学,2023,30(6):685-698. [17] 保长虹,李昭楠,关却多杰,等.青海湖裸鲤和花斑裸鲤线粒体基因组比较及其系统进化分析[J].华北农学报,2023,38(3):213-226. [18] 赵凯.青海湖及其邻近水系特有裂腹鱼类的分子系统发育及系统地理学[D].杨凌:西北农林科技大学,2005. [19] 迪丽娜·茹斯坦木.裂腹鱼亚科(Schizothoracinae)及鲃亚科(Barbinae)物种比较线粒体基因组学和系统发育研究[D].乌鲁木齐:新疆大学,2021. [20] TONG C, TANG Y T, ZHAO K. The complete mitochondrial genome of Gymnocypris przewalskii kelukehuensis (Teleostei:Cyprinidae)[J]. Conservation Genetics Resources,2017,9(3):443-445. [21] ZHANG Y, LI X H, TIAN F, et al. Mitochondrial genome and phylogenetic relationship of Gymnocypris eckloni (Schizothoracinae) in Qaidam river basin[J]. Genomics,2020,112(6):4316-4321. [22] 鲍鸣,奚业文,海思娆,等.麦鱼线粒体基因组序列测定与结构特征分析[J].河北渔业,2023(9):20-27. [23] 王思格,刘玉萍,朱子妍,等.前肛鳗线粒体基因组结构特征与系统发育关系研究[J].南方农业学报,2023,54(9):2512-2524. [24] TANAKA M, OZAWA T. Strand asymmetry in human mitochondrial DNA mutations [J]. Genomics,1994,22(2):327-335. [25] 邵爱华,杜建,陈葵,等.暗纹东方鲀线粒体基因组核苷酸全序列测定与分析[J].动物学杂志,2010,45(5):18-28. [26] SATOH M, HIRAYOSHI K, YOKOTA S, et al. Intracellular interaction of collagen-specific stress protein HSP47 with newly synthesized procollagen[J]. The Journal of Cell Biology,1996,133(2):469-483. [27] QIN Q, CHEN L, ZHANG F B, et al. Characterization of the complete mitochondrial genome of Schizothorax kozlovi (Cypriniformes, Cyprinidae, Schizothorax) and insights into the phylogenetic relationships of Schizothorax[J]. Animals,2024,14(5):721. [28] RUDOLF MEIER K S. DNA barcoding and taxonomy in Diptera:a tale of high intraspecific variability and low identification success[J]. Systematic Biology,2006,55(5):715-728. [29] COLLINS R A, CRUICKSHANK R H. The seven deadly sins of DNA barcoding[J]. Molecular Ecology Resources,2013,13(6):969-975. [30] 张亚颖,何慧,马海霞,等.小头高原鱼和骨唇黄河鱼线粒体基因组比较及系统发育分析[J].动物学杂志,2024,59(5):743-753. [31] 牛依萌,刘思嘉,田菲,等.渭河高原鳅线粒体基因组特征及其系统发育分析[J].基因组学与应用生物学,2024,43(增刊2):1813-1825. [32] 梁阳阳,张国庆,陈诚,等.须鳗虾虎鱼线粒体全基因组测序和系统发育分析[J].大连海洋大学学报,2023,38(5):772-778. [33] 李亚莉.青藏高原三种裂腹鱼线粒体全基因组的测定及分子进化分析[D].上海:复旦大学,2012. [34] 张艳萍,杜岩岩,娄忠玉,等.甘肃省几种裂腹鱼类系统发育关系探讨[J].西北师范大学学报(自然科学版),2013,49(5):91-96. [35] 武云飞,谭齐佳.青藏高原鱼类区系特征及其形成的地史原因分析[J].动物学报,1991(2):135-152. [36] 陈宜瑜,陈毅峰,刘焕章.青藏高原动物地理区的地位和东部界线问题[J].水生生物学报,1996,20(2):97-103. [37] 乔慧莹.基于线粒体基因序列分析裂腹鱼亚科的系统进化关系及中国沿海银鲳群体的遗传结构[D].上海:上海海洋大学,2014.
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