Peripheral Blood and Hematopoiesis Observation in Aspiorhynchus laticeps
FU Hongmei1,2,3, YANG Jie1, NIE Zhulan1,2,3, WEI Jie1,2,3, AI Tao3, XIAO Qing1,2,3
1. College of Life Science and Technology, Tarim University, Alaer 843300, China; 2. State Key Laboratory Incubation Base for Conservation and Utilization of Bio-Resource in Tarim Basin, Alaer 843300, China; 3. General Station of Aquaculture Technology Extension, Xinjiang Production and Construction Corps, Urumqi 830000, China
Abstract：To investigate the composition of blood cells and the process of hematopoiesis in fish Aspiorhynchus laticeps, and to provide insights into its ecological adaptation and supporting conservation efforts, peripheral blood smears and tissue imprints of kidney, spleen, and liver were prepared from A. laticeps,with body weight of(5.67±1.45) g, followed by Wright′s staining to analyze blood cell morphology, size, and proportions. Erythrocytes were found to be predominated in peripheral blood, and to exhibit elliptical or elongated cytoplasmic and nuclear morphology. The cytoplasm appeared to be pale red, with cell dimensions of (14.46±1.33) μm (major axis) and (8.44±0.73) μm (minor axis). Nuclei were centrally located, blue-purple in color, measuring (7.03±0.62) μm (major axis) and (3.43±0.32) μm (minor axis). Leukocytes included lymphocytes, thrombocytes, monocytes, and granulocytes, with the maximal proportion (88.09%) of lymphocytes. Hematopoiesis proceeded through primitive, immature, and mature stages, encompassing four lineages: erythrocytic, lymphocytic, monocytic, and granulocytic. Among hematopoietic organs, the kidney exhibited the maximal proportion of immature cells (30.82%), while the minimal activity (14.41%) was observed in hepatopancreas. The large erythrocyte size suggests that A. laticeps represents a phylogenetically primitive fish species. The kidney serves as the primary hematopoietic organ, with supplementary hemopoietic capacity observed in the spleen and hepatopancreas.
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2025, Vol. 44 
