DIA Quantitative Proteomics Analysis of Muscles in Anadromous and Landlocked Forms of Masu Salmon
JIAO Siqi1, YIN Zhuang2, LI Min1, HAO Yongkun1, KONG Yidi1, QU Guijuan1, FANG Qiongya1, WANG Guiqin1
1. Laboratory of Animal Production and Quality Security, Ministry of Education, College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China; 2. Mijiang Township Comprehensive Service Center of Hunchun City, Yanbian 133302, China
Abstract：In order to explore the adaptation mechanisms of two ecological types of masu salmon Oncorh ynchus masou, the muscle proteomes of anadromous (age 4, sexually mature females) and landlocked (age 2, sexually mature females) forms of masu salmon were comparcd by DIA based quatitative proteomics. A total of 737 differentially expressed proteins (DEPs) were identified (FC > 2, P < 0.05), including 324 upregulated and 413 downregulated proteins. GO function annotations revealed that DEPs were significantly associated with energy metabolism, lipid transport, fatty acid binding, oxidative stress regulation, and protein synthesis. In the anadromous type, proteins related to energy metabolism, oxidative stress defense, and protein biosynthesis were upregulated, suggesting enhanced energy supply, antioxidant capacity, and translational activity. Conversely, proteins involved in lipid transport and fatty acid binding were downregulated, indicating metabolic shift favoring lipid utilization in the landlocked type. KEGG pathway enrichment (starch and sucrose metabolism, purine metabolism, glycolysis/gluconeogenesis, glutathione metabolism) further highlighted the anadromous fish′s reliance on carbohydrate-based rapid energy production. Key protein changes, such as fatty acid-binding proteins, glutathione peroxidases, and mitochondrial oxidative phosphorylation components, reflect distinct genetic-metabolic regulation, antioxidant defense, and energy efficiency strategies. In summary, the anadromous ecotype exhibits muscle adaptations supporting high energy demands and environmental stress during long-distance migration and reproduction, while the landlocked ecotype demonstrates lipid metabolic adaptations to cold freshwater habitats.
焦思琦, 尹壮, 李民, 郝永坤, 孔祎頔, 曲桂娟, 方琼亚, 王桂芹. 洄游型与陆封型马苏大麻哈鱼肌肉的DIA定量蛋白组学分析[J]. 水产科学, 2026, 45(1): 36-44.
JIAO Siqi, YIN Zhuang, LI Min, HAO Yongkun, KONG Yidi, QU Guijuan, FANG Qiongya, WANG Guiqin. DIA Quantitative Proteomics Analysis of Muscles in Anadromous and Landlocked Forms of Masu Salmon. Fisheries Science, 2026, 45(1): 36-44.
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2026, Vol. 45 
