Mitochondrial Genome Sequencing and Comparative Analysis of Hermit Crab Pagurus ochotensis
BAO Xiangbo, TIAN Meilin, WANG Yangyang, LIU Weidong, LIU Zhongying
Key Laboratory of Protection and Utilization of Aquatic Germplasm Resource, Ministry of Agriculture and Rural Affairs, Liaoning Key Lab of Marine Fishery Molecular Biology, Liaoning Ocean and Fisheries Science Research Institute, Dalian 116023, China
Abstract：In order to clarify and organize classification system in Paguroidea with rich in species and numerous in quantity, as an economically important marine crab species in the coastal areas of Liaoning,the complete mitochondrial genome was sequenced, assembled, and annotated in hermit crab Pagurus ochotensis collected at Zhangzi Island waters, Dalian (E 122°80′77, N 38°98′86), Liaoning province using next-generation sequencing technology. The mitogenome is found to be 16 280 bp in length, containing 13 protein-coding genes (PCGs), 22 tRNAs, 2 rRNAs, and an AT-rich control region. There is AT content of 72.2% in genome, with an AT-skew of -0.006 and GC-skew of -0.144. Ten gene rearrangements were identified, including tRNA positional variations that showed remarkable intrageneric diversity. A Bayesian phylogenetic tree based on 13 PCGs revealed that the members in Paguroidea were clustered into two major clades, one comprising Coenobitidae and Diogenidae, and the other including Paguridae and Lithodidae. Comparative analysis indicated that members in marine groups (Paguridae and Lithodidae) had significantly higher AT contents than members in terrestrial/semi-terrestrial groups (Coenobitidae and Diogenidae) did, indicating that AT bias may be influenced by environmental stability and functional constraints. These findings enhance our understanding of the phylogenetic relationships within Paguroidea and the evolutionary dynamics of mitochondrial genomes in crustaceans.
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2026, Vol. 45 
