Seasonal Changes in Fish Community in Jinjiang River Based on Environmental DNA Metabarcoding
LI Sihui1, DENG Weide2, ZOU Lu3, SUN Zhenzhu2, LI Yuan2, XU Youhou1, WEN Jing2,4
1. College of Marine Science, Beibu Gulf University, Qinzhou 535011, China; 2. School of Biology and Agriculture, Shaoguan University, Shaoguan 512005, China; 3. Xining Customs Technical Center, Qinghai, Xining 810000, China; 4. Guangdong Provincial Key Laboratory of Utilization and Conservation of Food and Medicinal Resources in Northern Region, Shaoguan University, Shaoguan 512005, China
Abstract：Jinjiang River is the main branch of the Pearl River system. The Jinjiang River flows through Danxia, which is honored with Global Geoparks, World Heritage, China National Park, National Nature Reserve and National AAAAA Tourist Attractions.In order to understand the fish species diversity of Jinjiang River and Danxiashan in the Pearl River system, mixed water samples were collected from surface and bottom at 15 survey stations in Jinjiang River as the main tributary of the Pearl River system in November 2023, February, May and August 2024, and the changes in fish community in the four seasons were monitored by eDNA macro barcodes. The results showed that 2 622 108 reads, 99 fish species, were obtained for fish from 180 samples of 15 survey stations after bioinformatics filtering, which belong to 12 orders 21 families 69 genera, without significant difference in α diversity index among the different types of waters between Danxiashan and Non-Danxiashan. There was significant difference in fish community structure between spring and autumn, and no significant difference among other seasons. Seven invasive alien species were found, especially large number of Coptodon zillii in all survey stations in four seasons. The second-class national protected animal Hemibagrus guttatus was observed in 10 survey stations in four seasons. The findings provide detailed data on fish diversity and important implications for the long-term management and conservation of the Jinjiang river, and prove that eDNA has great potential in the research of fish diversity in river systems.
李斯惠, 邓维德, 邹璐, 孙珍珠, 黎源, 许尤厚, 文菁. 基于环境DNA宏条形码的锦江鱼类群落季节性研究[J]. 水产科学, 2025, 44(5): 695-706.
LI Sihui, DENG Weide, ZOU Lu, SUN Zhenzhu, LI Yuan, XU Youhou, WEN Jing. Seasonal Changes in Fish Community in Jinjiang River Based on Environmental DNA Metabarcoding. Fisheries Science, 2025, 44(5): 695-706.
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2025, Vol. 44 
